Jin C S, Ukai K, Sakakura Y
Nihon Jibiinkoka Gakkai Kaiho. 1989 May;92(5):716-21. doi: 10.3950/jibiinkoka.92.716.
To explain of the mechanism of the enhanced nasal epithelial permeability to HRP in patients with nasal allergy, the inferior turbinate mucosa was removed from 6 normal adults and 7 adults with nasal allergy. Difference of the fine structure of the intercellular junction was compared between normal mucosa and mucosa of nasal allergy by electron microscope. Staining pattern of four kinds of HRP-conjugated lectin (HRP-WGA, PNA, UEA-I and RCA-I) was also studied by electron microscope. There was no significant difference in the intercellular space of the mucosa between the normal mucosa and mucosa of nasal allergy. In the epithelial cell membrane, pattern of HRP-lectin staining was almost similar in both groups. In normal nasal epithelium, the intercellular junction consisted of junctional complex; adherent junction, desmosome and gap junction. The intercellular space was approximately 150-250 A in width. The tight junction was located beneath the luminal surface of the epithelium, and belt-like continuation connecting the adjacent cells. It was concluded that enhanced permeability to HRP in nasal allergy was not morphologic changes of the intercellular junction and component and distribution of the glycoconjugates in epithelial cellular membrane, but this may be based on functional changes.
为了解释鼻过敏患者鼻上皮对辣根过氧化物酶（HRP）通透性增强的机制，从6名正常成年人和7名鼻过敏成年人身上取下下鼻甲黏膜。通过电子显微镜比较正常黏膜和鼻过敏黏膜细胞间连接的细微结构差异。还通过电子显微镜研究了四种辣根过氧化物酶结合凝集素（HRP-WGA、PNA、UEA-I和RCA-I）的染色模式。正常黏膜和鼻过敏黏膜的细胞间隙没有显著差异。在上皮细胞膜中，两组的HRP-凝集素染色模式几乎相似。在正常鼻上皮中，细胞间连接由连接复合体组成；黏着连接、桥粒和缝隙连接。细胞间隙宽度约为150-250埃。紧密连接位于上皮腔面下方，是连接相邻细胞的带状连续结构。得出的结论是，鼻过敏中对HRP通透性增强不是细胞间连接以及上皮细胞膜中糖缀合物的成分和分布的形态学变化，而是可能基于功能变化。
