Masuyama K, Samejima Y, Ishikawa T
Department of Otorhinolaryngology, Kumamoto University, School of Medicine, Japan.
Acta Otolaryngol Suppl. 1988;458:181-9. doi: 10.3109/00016488809125126.
In order to understand the migration mechanism and character of eosinophils in the nose, the eosinophils in nasal mucosa and secretion were observed morphologically and physicochemically. In our electron microscopic observation of animal and human nasal mucosa, eosinophils migrated through the intercellular space, projecting pseudopods into the apical region of the space and splitting the junctions between epithelial cells. However, a freeze-fracture study in the experimental animals showed that the morphology of tight junction was not significantly changed except for slight decrease in the number of strands in antigen challenged animals. Eosinophilotactic activity was clearly demonstrated in nasal secretion of patients with allergic rhinitis. The factor in nasal secretion was a molecule smaller than 10,000 and sensitive to heating in higher degree. Additionally, the eosinophils in nasal secretion were hypodense, implying that they may be in activated state.
为了解嗜酸性粒细胞在鼻腔中的迁移机制和特性，对鼻黏膜及分泌物中的嗜酸性粒细胞进行了形态学和理化观察。在我们对动物和人类鼻黏膜的电子显微镜观察中，嗜酸性粒细胞通过细胞间隙迁移，将伪足伸入间隙的顶端区域并分离上皮细胞之间的连接。然而，对实验动物的冷冻断裂研究表明，除了抗原激发动物中连接丝数量略有减少外，紧密连接的形态没有明显变化。在变应性鼻炎患者的鼻腔分泌物中明确显示出嗜酸性粒细胞趋化活性。鼻腔分泌物中的趋化因子是一种分子量小于10,000且对热更敏感的分子。此外，鼻腔分泌物中的嗜酸性粒细胞密度较低，这意味着它们可能处于激活状态。
