DPY30在胃癌细胞增殖和运动中的作用
Roles of DPY30 in the Proliferation and Motility of Gastric Cancer Cells.
作者信息
Lee Yong Joo, Han Myoung-Eun, Baek Su-Jin, Kim Seon-Young, Oh Sae-Ock
机构信息
Departments of Anatomy, School of Medicine, Pusan National University, Busan, Republic of Korea.
Departments of Anatomy, School of Medicine, Pusan National University, Busan, Republic of Korea; Medical Research Center for Ischemic Tissue Regeneration, Pusan National University, Busan, Republic of Korea.
出版信息
PLoS One. 2015 Jul 6;10(7):e0131863. doi: 10.1371/journal.pone.0131863. eCollection 2015.
Various types of histone methylation have been associated with cancer progression. Depending on the methylation site in histone proteins, its effects on transcription are different. DPY30 is a common member of SET1/MLL histone H3K4 methyltransferase complexes. However, its expression and roles in gastric cancer have been poorly characterized. To determine whether DPY30 has pathophysiological roles in gastric cancer, its expression and roles were examined. Immunohistochemistry and real time PCR showed up-regulation of DPY30 expression in some gastric cancer cell lines and patients' tissues. Its knockdown by siRNA decreased the proliferation, migration, and invasion of gastric cancer cells, whereas its overexpression showed the opposite effects. These results indicate that DPY30 has critical roles in the proliferation, migration, and invasion of gastric cancer cells, and suggest DPY30 might be a therapeutic target in gastric cancer.
多种类型的组蛋白甲基化与癌症进展相关。根据组蛋白上的甲基化位点不同，其对转录的影响也不同。DPY30是SET1/MLL组蛋白H3K4甲基转移酶复合物的一个常见成员。然而，其在胃癌中的表达及作用尚未得到充分表征。为了确定DPY30在胃癌中是否具有病理生理作用，对其表达及作用进行了研究。免疫组织化学和实时PCR结果显示，DPY30在一些胃癌细胞系和患者组织中表达上调。通过小干扰RNA敲低其表达可降低胃癌细胞的增殖、迁移和侵袭能力，而其过表达则产生相反的效果。这些结果表明，DPY30在胃癌细胞的增殖、迁移和侵袭中起关键作用，提示DPY30可能是胃癌的一个治疗靶点。
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