• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

染色质调节剂DPY30在结肠癌细胞葡萄糖代谢中的作用。

The role of chromatin modulator DPY30 in glucose metabolism of colorectal cancer cells.

作者信息

Mao Xiaomei, Yang Shiqin, Zhang Ye, Yang Huajun, Yan Danhong, Zhang Lingzhi

机构信息

Department of Medical Science and Technology, Suzhou Chien-Shiung Institute of Technology, Taicang, China.

School of Life Sciences, Xiamen University, Xiamen, China.

出版信息

Transl Cancer Res. 2024 Aug 31;13(8):4205-4218. doi: 10.21037/tcr-24-366. Epub 2024 Aug 16.

DOI:10.21037/tcr-24-366
PMID:39262496
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11385247/
Abstract

BACKGROUND

Colorectal cancer (CRC) is the third most common cancer worldwide and the second leading cause of cancer-related death. This study investigated the role of DPY30 in the development and progression of CRC cells, especially in the area of cellular glycolysis.

METHODS

HT29 control cells and DPY30 knockdown cells were collected for tandem mass tag (TMT) labeling quantitative proteomics analysis of cellular total proteins (n=3). To further assess the accuracy of the differential expression profile, representative genes were selected and confirmed by quantitative real-time polymerase chain reaction (qPCR) and western blot (WB). Glycolytic flux was studied by detecting the extracellular acidification rate (ECAR) using the Seahorse XFe96. In view of the vital role of DPY30 on the H3K4me3 level, chromatin immunoprecipitation (ChIP) assays were performed.

RESULTS

The results showed that the expression of HK1, a protein related to cellular glucose metabolism, was significantly down-regulated after DPY30 knockdown, while the expression of GSK3B was significantly increased. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated significant changes in several signaling pathways, with the PI3K-AKT signaling pathway being the most prominent. The data of Seahorse XFe96 revealed that DPY30 knockdown attenuated aerobic glycolysis. DPY30 knockdown repressed the establishment of H3K4me3 on promoters of , , and .

CONCLUSIONS

DPY30 promoted the glycolysis of CRC cells through two channels: influencing signaling pathways and gene transcription, thereby promoting the progression of CRC.

摘要

背景

结直肠癌（CRC）是全球第三大常见癌症，也是癌症相关死亡的第二大主要原因。本研究调查了DPY30在CRC细胞发生和发展中的作用，特别是在细胞糖酵解方面。

方法

收集HT29对照细胞和DPY30敲低细胞用于细胞总蛋白的串联质谱标签（TMT）标记定量蛋白质组学分析（n = 3）。为了进一步评估差异表达谱的准确性，选择代表性基因并通过定量实时聚合酶链反应（qPCR）和蛋白质免疫印迹（WB）进行验证。使用Seahorse XFe96通过检测细胞外酸化率（ECAR）来研究糖酵解通量。鉴于DPY30对H3K4me3水平的重要作用，进行了染色质免疫沉淀（ChIP）分析。

结果

结果表明，DPY30敲低后，与细胞葡萄糖代谢相关的蛋白HK1的表达显著下调，而GSK3B的表达显著增加。京都基因与基因组百科全书（KEGG）通路分析表明，几个信号通路发生了显著变化，其中PI3K-AKT信号通路最为突出。Seahorse XFe96的数据显示，DPY30敲低减弱了有氧糖酵解。DPY30敲低抑制了 、 和 启动子上H3K4me3的建立。

结论

DPY30通过影响信号通路和基因转录两个途径促进CRC细胞的糖酵解，从而促进CRC的进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/cbc0d019166e/tcr-13-08-4205-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/211c1d2f778c/tcr-13-08-4205-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/3255a5175644/tcr-13-08-4205-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/e5ab7a0aea6a/tcr-13-08-4205-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/c66af508ff31/tcr-13-08-4205-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/cbc0d019166e/tcr-13-08-4205-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/211c1d2f778c/tcr-13-08-4205-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/3255a5175644/tcr-13-08-4205-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/e5ab7a0aea6a/tcr-13-08-4205-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/c66af508ff31/tcr-13-08-4205-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/305b/11385247/cbc0d019166e/tcr-13-08-4205-f5.jpg

相似文献

1
The role of chromatin modulator DPY30 in glucose metabolism of colorectal cancer cells.染色质调节剂DPY30在结肠癌细胞葡萄糖代谢中的作用。
Transl Cancer Res. 2024 Aug 31;13(8):4205-4218. doi: 10.21037/tcr-24-366. Epub 2024 Aug 16.
2
DPY30 knockdown suppresses colorectal carcinoma progression via inducing Raf1/MST2-mediated apoptosis.DPY30基因敲低通过诱导Raf1/MST2介导的凋亡抑制结直肠癌进展。
Heliyon. 2024 Jan 20;10(3):e24807. doi: 10.1016/j.heliyon.2024.e24807. eCollection 2024 Feb 15.
3
DPY30 Promotes Proliferation and Cell Cycle Progression of Colorectal Cancer Cells via Mediating H3K4 Trimethylation.DPY30 通过介导 H3K4 三甲基化促进结直肠癌细胞的增殖和细胞周期进程。
Int J Med Sci. 2023 May 11;20(7):901-917. doi: 10.7150/ijms.80073. eCollection 2023.
4
DPY30 promotes colorectal carcinoma metastasis by upregulating ZEB1 transcriptional expression.DPY30通过上调ZEB1转录表达促进结直肠癌转移。
Cancer Cell Int. 2023 Dec 19;23(1):333. doi: 10.1186/s12935-023-03126-y.
5
Bisphenol A exposure causes testicular toxicity by targeting DPY30-mediated post-translational modification of PI3K/AKT signaling in mice.双酚A暴露通过靶向小鼠中DPY30介导的PI3K/AKT信号转导的翻译后修饰而导致睾丸毒性。
Ecotoxicol Environ Saf. 2022 Sep 15;243:113996. doi: 10.1016/j.ecoenv.2022.113996. Epub 2022 Aug 26.
6
DPY30 functions in glucose homeostasis via integrating activated histone epigenetic modifications.DPY30 通过整合激活的组蛋白表观遗传修饰在葡萄糖稳态中发挥作用。
Biochem Biophys Res Commun. 2018 Dec 9;507(1-4):286-290. doi: 10.1016/j.bbrc.2018.11.023. Epub 2018 Nov 15.
7
DPY30 regulates pathways in cellular senescence through ID protein expression.DPY30 通过 ID 蛋白表达调控细胞衰老途径。
EMBO J. 2013 Aug 14;32(16):2217-30. doi: 10.1038/emboj.2013.159. Epub 2013 Jul 19.
8
lncCPSET1 acts as a scaffold for MLL2/COMPASS to regulate Bmp4 and promote the formation of chicken primordial germ cells.lncCPSET1 作为 MLL2/COMPASS 的支架，调节 Bmp4 并促进鸡原始生殖细胞的形成。
Mol Genet Genomics. 2024 Mar 29;299(1):41. doi: 10.1007/s00438-024-02127-4.
9
The DPY30-H3K4me3 Axis-Mediated PD-L1 Expression in Melanoma.DPY30-H3K4me3轴介导黑色素瘤中PD-L1的表达
J Inflamm Res. 2022 Sep 26;15:5595-5609. doi: 10.2147/JIR.S377678. eCollection 2022.
10
Insulin gene enhancer protein 1 mediates glycolysis and tumorigenesis of gastric cancer through regulating glucose transporter 4.胰岛素基因增强子蛋白 1 通过调节葡萄糖转运蛋白 4 介导胃癌的糖酵解和肿瘤发生。
Cancer Commun (Lond). 2021 Mar;41(3):258-272. doi: 10.1002/cac2.12141. Epub 2021 Feb 11.

引用本文的文献

1
Phosphofructokinase-1 redefined: a metabolic hub orchestrating cancer hallmarks through multi-dimensional control networks.磷酸果糖激酶-1的重新定义：一个通过多维控制网络协调癌症特征的代谢枢纽。
J Transl Med. 2025 Aug 6;23(1):873. doi: 10.1186/s12967-025-06897-2.

本文引用的文献

1
Oridonin inhibits bladder cancer survival and immune escape by covalently targeting HK1.冬凌草甲素通过共价靶向己糖激酶1抑制膀胱癌的存活和免疫逃逸。
Phytomedicine. 2024 Apr;126:155426. doi: 10.1016/j.phymed.2024.155426. Epub 2024 Feb 7.
2
Intermittent Hypoxia Promotes TAM-Induced Glycolysis in Laryngeal Cancer Cells via Regulation of HK1 Expression through Activation of ZBTB10.间歇性低氧通过激活 ZBTB10 调控 HK1 表达促进喉癌细胞中 TAM 诱导的糖酵解。
Int J Mol Sci. 2023 Sep 30;24(19):14808. doi: 10.3390/ijms241914808.
3
Depleted hexokinase1 and lack of AMPKα activation favor OXPHOS-dependent energetics in retinoblastoma tumors.
己糖激酶 1 耗竭和 AMPKα 激活缺失有利于视网膜母细胞瘤肿瘤中依赖 OXPHOS 的能量代谢。
Transl Res. 2023 Nov;261:41-56. doi: 10.1016/j.trsl.2023.07.001. Epub 2023 Jul 6.
4
DPY30 Promotes Proliferation and Cell Cycle Progression of Colorectal Cancer Cells via Mediating H3K4 Trimethylation.DPY30 通过介导 H3K4 三甲基化促进结直肠癌细胞的增殖和细胞周期进程。
Int J Med Sci. 2023 May 11;20(7):901-917. doi: 10.7150/ijms.80073. eCollection 2023.
5
Disease Burden of Total and Early-Onset Colorectal Cancer in China from 1990 to 2019 and Predictions of Cancer Incidence and Mortality.1990年至2019年中国总体及早发性结直肠癌的疾病负担以及癌症发病率和死亡率预测
Clin Epidemiol. 2023 Feb 1;15:151-163. doi: 10.2147/CLEP.S391058. eCollection 2023.
6
HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma.HK1 来源于肝星状细胞衍生的细胞外囊泡，促进了肝细胞癌的进展。
Nat Metab. 2022 Oct;4(10):1306-1321. doi: 10.1038/s42255-022-00642-5. Epub 2022 Oct 3.
7
Artesunate Inhibits the Development of Esophageal Cancer by Targeting HK1 to Reduce Glycolysis Levels in Areas With Zinc Deficiency.青蒿琥酯通过靶向HK1降低缺锌区域的糖酵解水平来抑制食管癌的发展。
Front Oncol. 2022 May 12;12:871483. doi: 10.3389/fonc.2022.871483. eCollection 2022.
8
YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect.YTHDC1 通过减弱 RUNX1 诱导的糖酵解激活转录来增强 miR-30d 抑制胰腺肿瘤发生。
Cell Death Differ. 2021 Nov;28(11):3105-3124. doi: 10.1038/s41418-021-00804-0. Epub 2021 May 21.
9
Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.《全球癌症统计数据 2020：全球 185 个国家和地区 36 种癌症的发病率和死亡率估计》。
CA Cancer J Clin. 2021 May;71(3):209-249. doi: 10.3322/caac.21660. Epub 2021 Feb 4.
10
A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma.A20 靶向 PFKL 和糖酵解以抑制肝细胞癌的进展。
Cell Death Dis. 2020 Feb 3;11(2):89. doi: 10.1038/s41419-020-2278-6.