Department of Inorganic and Organic Chemistry, Faculty of Experimental Sciences, Campus of International Excellence in Agri-Food (ceiA3), University of Jaén, 23071 Jaén, Spain.
Institute of Biopathology and Regenerative Medicine, University of Granada, 18071 Granada, Spain.
Molecules. 2024 Aug 16;29(16):3887. doi: 10.3390/molecules29163887.
The Japanese plum tree ( Lindl.) is mainly cultivated in temperate areas of China and some European countries. Certain amounts of wood (from pruning works) are generated every year from this crop of worldwide commercial significance. The main objective of this work was to value this agricultural woody residue, for which the chemical composition of pruning wood extracts from six Japanese plum cultivars was investigated, and the antiproliferative activity of extracts and pure phenolics present in those extracts was measured. For the chemical characterization, total phenolic content and DPPH radical-scavenging assays and HPLC‒DAD/ESI‒MS analyses were performed, with the procyanidin (-)-ent-epicatechin-(2α→→7,4α→8)-epicatechin () and the propelargonidin (+)-epiafzelechin-(2β→→7,4β→8)-epicatechin () being the major components of the wood extracts. Some quantitative differences were found among plum cultivars, and the content of proanthocyanidins ranged from 1.50 (cv. 'Fortune') to 4.44 (cv. 'Showtime') mg/g of dry wood. Regarding the antitumoral activity, eight wood extracts and four phenolic compounds were evaluated in MCF-7 cells after 48 h of induction, showing the wood extract from cv. 'Songold' and (‒)-annphenone (), the best antiproliferative activity (IC: 424 μg/mL and 405 μg/mL, respectively).
日本李树（Lindl.）主要在中国和一些欧洲国家的温带地区种植。每年从这种具有全球商业意义的作物中都会产生一定数量的木材（来自修剪工作）。这项工作的主要目的是评估这种农业木质残余物的价值，为此研究了六个日本李品种修剪木提取物的化学成分，并测量了提取物和存在于这些提取物中的纯酚类物质的抗增殖活性。对于化学特性，进行了总酚含量和 DPPH 自由基清除测定以及 HPLC-DAD/ESI-MS 分析，其中原花青素（-）-ent-表儿茶素-（2α→→7,4α→8）-表儿茶素（）和原花青素（+）-epiafzelechin-（2β→→7,4β→8）-表儿茶素（）是木材提取物的主要成分。在李品种之间发现了一些定量差异，原花青素的含量范围为 1.50（cv. 'Fortune'）至 4.44（cv. 'Showtime'）mg/g 干木。关于抗肿瘤活性，在 MCF-7 细胞中诱导 48 小时后，评估了八种木材提取物和四种酚类化合物，结果表明 cv. 'Songold'的木材提取物和（-）-annphenone（）具有最佳的抗增殖活性（IC：424 μg/mL 和 405 μg/mL）。
