Burns C P, Wagner B A, North J A
Department of Medicine, University of Iowa College of Medicine, Iowa City 52242.
Med Oncol Tumor Pharmacother. 1989;6(4):245-53. doi: 10.1007/BF02985156.
Hyperthermia is used experimentally to treat human malignancy. The effect of heat delivery rate and thermotolerance on the expression of heat shock proteins (hsp) by the human HL-60 cell line before and after differentiation was studied. This leukemia cell synthesized multiple hsp in response to elevated temperatures. The most obvious and consistent proteins were within the highly conserved stress-inducible family of polypeptides hsp70 which resolved as a hsp69/72 doublet. Cells which were made thermotolerant by gradual heating selectively failed to express the hsp70 doublet even though other hsp were synthesized. Mature HL-60 cells induced to differentiate by incubation in retinoic acid expressed a full complement of hsp when exposed to immediate heat, but there was selective deletion of hsp70 with gradual hyperthermia. This model for selective induction of hsp confirms that synthesis of hsp and thermotolerance can be dissociated in the HL-60. It suggests that the hsp70 does not play an obligatory role in thermotolerance of this human leukemia cell.
高温疗法被用于人体恶性肿瘤的实验性治疗。研究了热传递速率和热耐受性对人HL-60细胞系分化前后热休克蛋白（hsp）表达的影响。这种白血病细胞在温度升高时会合成多种热休克蛋白。最明显且一致的蛋白存在于高度保守的应激诱导多肽家族hsp70中，其表现为hsp69/72双峰。通过逐渐加热产生热耐受性的细胞即使合成了其他热休克蛋白，也选择性地未能表达hsp70双峰。经视黄酸孵育诱导分化的成熟HL-60细胞在立即受热时表达完整的热休克蛋白，但在逐渐升温时hsp70会被选择性缺失。这种热休克蛋白选择性诱导模型证实，在HL-60细胞中热休克蛋白的合成与热耐受性可以分离。这表明hsp70在这种人类白血病细胞的热耐受性中不发挥必然作用。
