Nassar A, Darrasse A, Lemattre M, Kotoujansky A, Dervin C, Vedel R, Bertheau Y
Station de Pathologie Végétale, Institut National de la Recherche Agronomique, Versailles, France.
Appl Environ Microbiol. 1996 Jul;62(7):2228-35. doi: 10.1128/aem.62.7.2228-2235.1996.
Conserved regions about 420 bp long of the pelADE cluster specific to Erwinia chrysanthemi were amplified by PCR and used to differentiate 78 strains of E. chrysanthemi that were obtained from different hosts and geographical areas. No PCR products were obtained from DNA samples extracted from other pectinolytic and nonpectinolytic species and genera. The pel fragments amplified from the E. chrysanthemi strains studied were compared by performing a restriction fragment length polymorphism (RFLP) analysis. On the basis of similarity coefficients derived from the RFLP analysis, the strains were separated into 16 PCR RFLP patterns grouped in six clusters, These clusters appeared to be correlated with other infraspecific levels of E. chrysanthemi classification, such as pathovar and biovar, and occasionally with geographical origin. Moreover, the clusters correlated well with the polymorphism of pectate lyase and pectin methylesterase isoenzymes. While the pectin methylesterase profiles correlated with host monocot-dicot classification, the pectate lyase polymorphism might reflect the cell wall microdomains of the plants belonging to these classes.
通过PCR扩增了菊欧文氏菌特有的pelADE簇约420 bp长的保守区域，并用于区分从不同宿主和地理区域获得的78株菊欧文氏菌。从其他果胶分解和非果胶分解的种属中提取的DNA样品未获得PCR产物。通过进行限制性片段长度多态性（RFLP）分析，比较了从所研究的菊欧文氏菌菌株中扩增的pel片段。根据RFLP分析得出的相似系数，这些菌株被分为16种PCR RFLP模式，归为6个簇，这些簇似乎与菊欧文氏菌分类的其他种下水平相关，如致病型和生化型，偶尔也与地理来源相关。此外，这些簇与果胶酸裂解酶和果胶甲酯酶同工酶的多态性密切相关。虽然果胶甲酯酶谱与宿主单子叶-双子叶分类相关，但果胶酸裂解酶多态性可能反映了属于这些类别的植物的细胞壁微区。
