Takahashi Fuminori, Kurokawa Hiroyasu, Shibasaki Sho, Kawamoto Ryo, Murayama Ryosuke, Miyazaki Masashi
a Department of Operative Dentistry, Nihon University School of Dentistry , Chiyoda-ku, Tokyo, Japan.
Acta Odontol Scand. 2016;74(2):142-7. doi: 10.3109/00016357.2015.1066850. Epub 2015 Jul 9.
This study evaluates the effect of self-assembling peptide P11-4 (Curodont Repair, CDR) on bovine enamel remineralization by measuring changes in ultrasonic propagation velocity.
Six specimens per group were prepared by sectioning bovine teeth into enamel blocks. These blocks were then immersed in lactic acid buffer solution (pH = 4.75) for 10 min twice a day and stored in artificial saliva. Other specimens were first treated with CDR, followed by a 10-min immersion in the lactic acid buffer solution twice a day, before storage in artificial saliva. The propagation time of longitudinal ultrasonic waves was measured using a pulser/receiver. Six specimens were used for each treatment protocol. The obtained data were statistically analyzed using ANOVA followed by Tukey's honestly significant difference tests (α = 0.05). Specimens were observed using laser scanning microscopy and scanning electron microscopy.
Sonic velocity was found to decrease with time for specimens stored in the demineralizing solution. On the other hand, increases in sonic velocity were found for specimens treated with CDR. These specimens also exhibited signs of mineral deposition.
By measuring the ultrasonic propagation velocity, it can be concluded that CDR application has an ability to promote bovine enamel remineralization.
本研究通过测量超声传播速度的变化，评估自组装肽P11-4（Curodont修复剂，CDR）对牛牙釉质再矿化的影响。
将牛牙切成牙釉质块，每组制备6个标本。然后将这些牙釉质块每天两次浸泡在乳酸缓冲溶液（pH = 4.75）中10分钟，并保存在人工唾液中。其他标本先用CDR处理，然后每天两次浸泡在乳酸缓冲溶液中10分钟，再保存在人工唾液中。使用脉冲发生器/接收器测量纵向超声波的传播时间。每个处理方案使用6个标本。所获得的数据使用方差分析，随后进行Tukey真实显著差异检验（α = 0.05）进行统计分析。使用激光扫描显微镜和扫描电子显微镜观察标本。
发现保存在脱矿溶液中的标本声速随时间降低。另一方面，发现用CDR处理的标本声速增加。这些标本还表现出矿物质沉积的迹象。
通过测量超声传播速度，可以得出结论，应用CDR有促进牛牙釉质再矿化的能力。
