Suda S, Takamizawa T, Takahashi F, Tsujimoto A, Akiba S, Nagura Y, Kurokawa H, Miyazaki M
Oper Dent. 2018 Jul/Aug;43(4):E166-E172. doi: 10.2341/17-175-L. Epub 2018 Mar 7.
The aim of this study was to use ultrasonography to evaluate the effect of the self-assembling peptide P11-4 on acid erosion prevention. Curodont Repair (CR), which includes peptide P11-4, was used. Rectangular prisms of bovine enamel (4×1×1 mm) were immersed in pure orange juice for a period of 5 minutes six times per day for 28 days. These samples were divided into four groups of six specimens each and treated differently for an additional period of 28 days: 1) baseline group specimens were stored in artificial saliva; 2) CR group specimens were exposed to curodont without acid challenge; 3) NCRA (no curodont+acid challenge) specimens were treated with orange juice without curodont exposure; and 4) CRA (CR+acid challenge) specimens were treated with curodont before treatment with orange juice. The propagation time of longitudinal ultrasonic velocity (UV) was measured. Ultrastructural observation of each tested enamel surface was carried out using field-emission scanning electron microscopy (SEM). The UV data were analyzed using two-way analysis of variance with time and treatment as confounding factors. Post hoc pairwise tests among groups were performed using the Tukey honestly significant difference test. The average UV in intact bovine enamel for the baseline group ranged from 4,483 to 4,549 m/s and did not vary significantly within the test period. The average ultrasonic velocity (UV) in all samples decreased after the initial erosion. The UV in NCRA decreased further over time. Increased UVs were found for CR and CRA. For CR and CRA, there was no significant difference in UV at the end of the experiment from the initial value before erosion. In the results of SEM observation, the CR and CRA groups had similar morphologic features in that etching patterns were not clearly due to precipitation between the enamel rods. From the results of this in vitro study, it might be concluded that applying enamel matrix derivatives and self-assembling peptides on erosive lesions can improve remineralization.
本研究的目的是利用超声检查来评估自组装肽P11-4对预防酸蚀的效果。使用了包含肽P11-4的Curodont Repair（CR）。将牛牙釉质的长方体（4×1×1毫米）每天6次浸入纯橙汁中5分钟，持续28天。这些样本被分成四组，每组六个标本，并在另外28天内进行不同处理：1）基线组标本保存在人工唾液中；2）CR组标本暴露于Curodont但无酸刺激；3）NCRA（无Curodont+酸刺激）标本用橙汁处理但未暴露于Curodont；4）CRA（CR+酸刺激）标本在用橙汁处理前先用Curodont处理。测量纵向超声速度（UV）的传播时间。使用场发射扫描电子显微镜（SEM）对每个测试的牙釉质表面进行超微结构观察。将UV数据作为混杂因素，采用时间和处理的双向方差分析进行分析。组间的事后两两检验使用Tukey真实显著差异检验进行。基线组完整牛牙釉质的平均UV范围为4483至4549米/秒，在测试期内无显著变化。所有样本在初始侵蚀后平均超声速度（UV）均下降。NCRA中的UV随时间进一步下降。CR和CRA的UV增加。对于CR和CRA，实验结束时的UV与侵蚀前的初始值无显著差异。在SEM观察结果中，CR组和CRA组具有相似的形态特征，即由于牙釉柱之间的沉淀，蚀刻图案不明显。从这项体外研究的结果可以得出结论，在侵蚀性病变上应用牙釉质基质衍生物和自组装肽可以改善再矿化。
