Danisman Hikmetnur, Delikan Ebru, Orhan Kaan
Department of Orthodontics, School of Dentistry, University of Nuh Naci Yazgan, Kayseri, Turkey.
Department of Oral and Maxillofacial Radiology, School of Dentistry, University of Ankara, Ankara, Turkey.
Sci Rep. 2025 Apr 3;15(1):11512. doi: 10.1038/s41598-025-95781-6.
The aim of this study was to assess the efficacy of the self-assembling peptide P11-4 [Curodont Repair Fluoride Plus-self-assembling peptide (Curodont FP)] and casein phosphopeptide-amorphous calcium phosphate fluoride (CCP-ACPF) (MI Varnish) in the management of white spot lesions (WSLs), during orthodontic treatment. Eighty specimens were prepared from the buccal surfaces of bovine teeth and divided into five groups that received different treatment protocols with Curodont FP and MI Varnish. (1) MI- Pre-Post: MI varnish applied before and after demineralization; (2) MI-Post: MI varnish applied after demineralization; (3) Curodont- Pre-Post: Curodont FP applied before and after demineralization; (4) Curodont-Post: Curodont FP applied after demineralization; (5) Control: No remineralization agent applied after demineralization. MI Varnish was applied in a thin and uniform layer using a single-use brush. Before application of Curodont FP, the enamel surface was conditioned with 35% phosphoric acid, rinsed and gently air-dried, then applied with an applicator sponge. Generalized linear models and one-way ANOVA tests were used to analyze the data. The significance level was set at p < 0.05. Specimens were analyzed using micro-computed tomography (micro-CT), field-emission scanning electron microscopy (FE-SEM), and energy-dispersive X-ray spectroscopy (EDX). The lowest lesion depths after the 28-day period (T2) were observed in the MI-Pre-Post (0.14 mm), MI-Post (0.15 mm), and Curodont-Pre-Post (0.15 mm) groups (p = 0.003). The control group showed the lowest mineral density (2.28 gHA/cm), while the MI-Pre-Post, MI-Post, and Curodont-Pre-Post groups exhibited the highest mineral density (2.54 gHA/cm, 2.52 gHA/cm, 2.56 gHA/cm, respectively) and the lowest lesion depth. Lesion depth and mineral density notably improved over time, particularly after agent application. The application of MI varnish before orthodontic treatment protects the enamel against demineralization. MI Varnish and Curodont FP are highly effective for the remineralization of WSL and can be clinically preferred.
本研究旨在评估自组装肽P11-4[Curodont修复氟化物加自组装肽（Curodont FP）]和酪蛋白磷酸肽-无定形磷酸钙氟化物（CCP-ACPF）（MI涂剂）在正畸治疗期间对白斑病变（WSL）的治疗效果。从牛牙颊面制备80个标本，分为五组，分别接受不同的Curodont FP和MI涂剂治疗方案。（1）MI-预处理-后处理组：脱矿前后均应用MI涂剂；（2）MI-后处理组：脱矿后应用MI涂剂；（3）Curodont-预处理-后处理组：脱矿前后均应用Curodont FP；（4）Curodont-后处理组：脱矿后应用Curodont FP；（5）对照组：脱矿后不应用再矿化剂。使用一次性刷子将MI涂剂涂成薄而均匀的一层。在应用Curodont FP之前，用35%的磷酸对牙釉质表面进行预处理，冲洗并轻轻吹干，然后用涂抹海绵涂抹。使用广义线性模型和单因素方差分析来分析数据。显著性水平设定为p<0.05。使用微型计算机断层扫描（micro-CT）、场发射扫描电子显微镜（FE-SEM）和能量色散X射线光谱仪（EDX）对标本进行分析。在28天（T2）后，MI-预处理-后处理组（0.14mm）、MI-后处理组（0.15mm）和Curodont-预处理-后处理组（0.15mm）的病变深度最低（p=0.003）。对照组的矿物质密度最低（2.28 gHA/cm），而MI-预处理-后处理组、MI-后处理组和Curodont-预处理-后处理组的矿物质密度最高（分别为2.54 gHA/cm、2.52 gHA/cm、2.56 gHA/cm），病变深度最低随着时间的推移，病变深度和矿物质密度显著改善，尤其是在应用药物后。正畸治疗前应用MI涂剂可保护牙釉质防止脱矿。MI涂剂和Curodont FP对WSL的再矿化非常有效，临床上可优先选用。
