新型隐球菌荚膜：来自光镊及其他生物物理工具应用的经验教训
The Cryptococcus neoformans capsule: lessons from the use of optical tweezers and other biophysical tools.
作者信息
Pontes Bruno, Frases Susana
机构信息
Laboratório de Pinças Óticas da Coordenação de Programas de Estudos Avançados, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro , Rio de Janeiro, Brazil.
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro , Rio de Janeiro, Brazil.
出版信息
Front Microbiol. 2015 Jun 24;6:640. doi: 10.3389/fmicb.2015.00640. eCollection 2015.
The fungal pathogen Cryptococcus neoformans causes life-threatening infections in immunocompromised individuals, representing one of the leading causes of morbidity and mortality in AIDS patients. The main virulence factor of C. neoformans is the polysaccharide capsule; however, many fundamental aspects of capsule structure and function remain poorly understood. Recently, important capsule properties were uncovered using optical tweezers and other biophysical techniques, including dynamic and static light scattering, zeta potential and viscosity analysis. This review provides an overview of the latest findings in this emerging field, explaining the impact of these findings on our understanding of C. neoformans biology and resistance to host immune defenses.
真菌病原体新型隐球菌可在免疫功能低下的个体中引发危及生命的感染，是艾滋病患者发病和死亡的主要原因之一。新型隐球菌的主要毒力因子是多糖荚膜；然而，荚膜结构和功能的许多基本方面仍知之甚少。最近，利用光镊和其他生物物理技术，包括动态和静态光散射、zeta电位和粘度分析，发现了荚膜的重要特性。本综述概述了这一新兴领域的最新发现，解释了这些发现对我们理解新型隐球菌生物学以及对宿主免疫防御的抗性的影响。
Zotero 插件