Onal Ozkan, Yetisir Fahri, Sarer A Ebru Salman, Zeybek N Dilara, Onal C Oztug, Yurekli Banu, Celik H Tugrul, Sirma Ayse, Kılıc Mehmet
Department of Anesthesiology and Reanimation, Selçuk University Medical Faculty, 42100 Konya, Turkey.
Department of General Surgery, Atatürk Education and Research Hospital, 06600 Ankara, Turkey.
Mediators Inflamm. 2015;2015:792016. doi: 10.1155/2015/792016. Epub 2015 Jun 16.
Intestinal ischemia-reperfusion injury is associated with mucosal damage and has a high rate of mortality. Various beneficial effects of ozone have been shown. The aim of the present study was to show the effects of ozone in ischemia reperfusion model in intestine.
Twenty eight Wistar rats were randomized into four groups with seven rats in each group. Control group was administered serum physiologic (SF) intraperitoneally (ip) for five days. Ozone group was administered 1 mg/kg ozone ip for five days. Ischemia Reperfusion (IR) group underwent superior mesenteric artery occlusion for one hour and then reperfusion for two hours. Ozone + IR group was administered 1 mg/kg ozone ip for five days and at sixth day IR model was applied. Rats were anesthetized with ketamine∖xyzlazine and their intracardiac blood was drawn completely and they were sacrificed. Intestinal tissue samples were examined under light microscope. Levels of superoxide dismutase (SOD), catalase (CAT), glutathioneperoxidase (GSH-Px), malondyaldehide (MDA), and protein carbonyl (PCO) were analyzed in tissue samples. Total oxidant status (TOS), and total antioxidant capacity (TAC) were analyzed in blood samples. Data were evaluated statistically by Kruskal Wallis test.
In the ozone administered group, degree of intestinal injury was not different from the control group. IR caused an increase in intestinal injury score. The intestinal epithelium maintained its integrity and decrease in intestinal injury score was detected in Ozone + IR group. SOD, GSH-Px, and CAT values were high in ozone group and low in IR. TOS parameter was highest in the IR group and the TAC parameter was highest in the ozone group and lowest in the IR group.
In the present study, IR model caused an increase in intestinal injury.In the present study, ozone administration had an effect improving IR associated tissue injury. In the present study, ozone therapy prevented intestine from ischemia reperfusion injury. It is thought that the therapeutic effect of ozone is associated with increase in antioxidant enzymes and protection of cells from oxidation and inflammation.
肠缺血再灌注损伤与黏膜损伤相关，死亡率高。已显示臭氧具有多种有益作用。本研究的目的是展示臭氧在肠缺血再灌注模型中的作用。
28只Wistar大鼠随机分为四组，每组7只。对照组腹腔注射生理血清（SF），持续5天。臭氧组腹腔注射1mg/kg臭氧，持续5天。缺血再灌注（IR）组进行肠系膜上动脉阻断1小时，然后再灌注2小时。臭氧 + IR组腹腔注射1mg/kg臭氧，持续5天，在第6天应用IR模型。用氯胺酮∖赛拉嗪麻醉大鼠，完全抽取其心内血后处死。在光学显微镜下检查肠组织样本。分析组织样本中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）和蛋白质羰基（PCO）的水平。分析血样中的总氧化剂状态（TOS）和总抗氧化能力（TAC）。数据采用Kruskal Wallis检验进行统计学评估。
在臭氧给药组中，肠损伤程度与对照组无差异。IR导致肠损伤评分增加。肠上皮保持完整，臭氧 + IR组肠损伤评分降低。臭氧组SOD、GSH-Px和CAT值高，IR组低。TOS参数在IR组中最高，TAC参数在臭氧组中最高，在IR组中最低。
在本研究中，IR模型导致肠损伤增加。在本研究中，臭氧给药对改善IR相关组织损伤有作用。在本研究中，臭氧疗法可预防肠缺血再灌注损伤。认为臭氧的治疗作用与抗氧化酶增加以及保护细胞免受氧化和炎症有关。
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