Kim Jiyeun Kate, Lee Jun Beom, Huh Ye Rang, Jang Ho Am, Kim Chan-Hee, Yoo Jin Wook, Lee Bok Luel
Department of Microbiology, Kosin University College of Medicine, Busan 602-703, South Korea.
Global Research Laboratory of Insect Symbiosis, College of Pharmacy, Pusan National University, Busan 609-735, South Korea.
Dev Comp Immunol. 2015 Nov;53(1):265-9. doi: 10.1016/j.dci.2015.07.006. Epub 2015 Jul 9.
The relation between gut symbiosis and immunity has been reported in various animal model studies. Here, we corroborate the effect of gut symbiont to host immunity using the bean bug model. The bean bug, Riptortus pedestris, is a useful gut symbiosis model due to the monospecific gut symbiont, genus Burkholderia. To examine the effect of gut symbiosis to host immunity, we generated the gut symbiont-harboring (symbiotic) insect line and the gut symbiont-lacking (aposymbiotic) insect line. Upon bacterial challenges, the symbiotic Riptortus exhibited better survival than aposymbiotic Riptortus. When cellular immunity was inhibited, the symbiotic Riptortus still survived better than aposymbioic Riptortus, suggesting stronger humoral immunity. The molecular basis of the strong humoral immunity was further confirmed by the increase of hemolymph antimicrobial activity and antimicrobial peptide expression in the symbiotic insects. Taken together, our data clearly demonstrate that Burkhoderia gut symbiont positively affect the Riptortus systemic immunity.
在各种动物模型研究中，已报道了肠道共生与免疫之间的关系。在此，我们使用豆蝽模型证实了肠道共生菌对宿主免疫的影响。豆蝽（Riptortus pedestris）由于其单一的肠道共生菌伯克霍尔德菌属，是一种有用的肠道共生模型。为了研究肠道共生对宿主免疫的影响，我们构建了携带肠道共生菌的（共生）昆虫品系和缺乏肠道共生菌的（无共生菌）昆虫品系。在受到细菌攻击时，共生的豆蝽比无共生菌的豆蝽表现出更好的存活率。当细胞免疫受到抑制时，共生的豆蝽仍然比无共生菌的豆蝽存活得更好，这表明其体液免疫更强。共生昆虫血淋巴抗菌活性和抗菌肽表达的增加进一步证实了强体液免疫的分子基础。综上所述，我们的数据清楚地表明，伯克霍尔德菌肠道共生菌对豆蝽的全身免疫有积极影响。
