Ostdiek A M, Ivey J R, Grant D A, Gopaldas J, Grant S A
Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.
Department of Biomedical Sciences, University of Missouri, Columbia, MO 65211, USA.
Biomaterials. 2015 Oct;65:175-83. doi: 10.1016/j.biomaterials.2015.06.045. Epub 2015 Jun 30.
Currently vascular repairs are treated using synthetic or biologic patches, however these patches have an array of complications, including calcification, rupture, re-stenosis, and intimal hyperplasia. An active patch material composed of decellularized tissue conjugated to gold nanoparticles (AuNPs) was developed and the long term biocompatibility and cellular integration was investigated. Porcine abdominal aortic tissue was decellularized and conjugated with 100 nm gold nanoparticles (AuNP). These patches were placed over a longitudinal arteriotomy of the thoracic aorta in six pigs. The animals were monitored for six months. Gross, histological, and immunohistochemical analyses of the patches were performed after euthanasia. Grossly there was minimal scar tissue with the patches still visible on the outer surface of the vessel. The inner lumen was smooth with a seamless transition from patch to native tissue. Histology demonstrated infiltration of host cells into the patch material. The immunohistochemical results demonstrated an endothelial cell layer forming over the patch within the vessel. Smooth muscle cells were repopulating the biomaterial in all animals. These results demonstrated that the AuNP biomaterial patch integrated well with the host tissue and did not failed over the six month implantation time.
目前，血管修复采用合成或生物补片进行治疗，然而这些补片存在一系列并发症，包括钙化、破裂、再狭窄和内膜增生。一种由脱细胞组织与金纳米颗粒（AuNPs）结合而成的活性补片材料被研发出来，并对其长期生物相容性和细胞整合情况进行了研究。将猪腹主动脉组织进行脱细胞处理，并与100纳米的金纳米颗粒（AuNP）结合。将这些补片放置在6头猪的胸主动脉纵行切开处。对这些动物进行了6个月的监测。在安乐死后对补片进行大体、组织学和免疫组织化学分析。大体观察发现瘢痕组织极少，补片仍可见于血管外表面。内腔光滑，从补片到天然组织无缝过渡。组织学显示宿主细胞浸润到补片材料中。免疫组织化学结果表明，血管内补片上形成了一层内皮细胞层。所有动物的生物材料中平滑肌细胞都在重新生长。这些结果表明，AuNP生物材料补片与宿主组织整合良好，在6个月的植入期内未出现失效情况。
