Salinas-Sánchez David Osvaldo, Zamilpa Alejandro, Pérez Salud, Herrera-Ruiz Maribel, Tortoriello Jaime, González-Cortazar Manasés, Jiménez-Ferrer Enrique
Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Xochitepec, Morelos, México.
Departamento de Sistemas Biológicos, UAM-Xochimilco, México, D. F., México.
Planta Med. 2015 Sep;81(14):1240-7. doi: 10.1055/s-0035-1546197. Epub 2015 Jul 10.
In the present work, the antiarthritic activity of hautriwaic acid is reported. This ent-clerodane diterpene isolated from Dodonaea viscosa was evaluated in mice using a kaolin/carrageenan-induced monoarthritis model. The inflammation observed in the joint (knee) on days 1-8 ranged from 50-70 %. After 10 days of treatment with different doses of hautriwaic acid (5, 10, 20 mg/kg), a decrease in knee inflammation was detected. This recovery was observed with both reference drugs, methotrexate (1 mg/kg) and diclofenac (0.75 mg/kg). In these groups of mice, the concentration of proinflammatory cytokines interleukin-1 beta, interleukin-6, and tumor necrosis factor alpha in the joint was significantly lower than that of the negative control group (animals with damage without any treatment). The negative control group presented a decrease in the concentration of interleukin-10, while the groups that received hautriwaic acid at different dose exhibited an increase in this interleukin. This anti-inflammatory cytokine was not modified in the joint of mice with diclofenac, but in mice that received methotrexate, a significant decrease was observed. Hautriwaic acid isolated from D. viscosa diminished the joint edema induced by this mixture of polysaccharides (carrageenan), possibly by acting as immunomodulator of the inflammatory response.
在本研究中，报道了 hautriwaic 酸的抗关节炎活性。从坡柳中分离得到的这种对映-克罗烷二萜在小鼠中使用高岭土/角叉菜胶诱导的单关节炎模型进行了评估。在第 1 - 8 天观察到关节（膝盖）处的炎症范围为 50 - 70%。在用不同剂量的 hautriwaic 酸（5、10、20 毫克/千克）治疗 10 天后，检测到膝盖炎症有所减轻。使用两种对照药物甲氨蝶呤（1 毫克/千克）和双氯芬酸（0.75 毫克/千克）时也观察到了这种恢复情况。在这些小鼠组中，关节中促炎细胞因子白细胞介素 -1β、白细胞介素 -6 和肿瘤坏死因子α的浓度显著低于阴性对照组（未接受任何治疗的损伤动物）。阴性对照组白细胞介素 -10 的浓度降低，而接受不同剂量 hautriwaic 酸的组中这种白细胞介素有所增加。在使用双氯芬酸治疗的小鼠关节中，这种抗炎细胞因子没有变化，但在接受甲氨蝶呤治疗的小鼠中，观察到其显著降低。从坡柳中分离得到的 hautriwaic 酸可能通过作为炎症反应的免疫调节剂，减轻了由这种多糖混合物（角叉菜胶）诱导的关节水肿。
