Gao Jun-Xue, Pei Qiu-Yan, Li Yun-Tao, Yang Zhen-Juan
Ultrasound Department, Peking University People's Hospital, Beijing, China.
Ultrasound Department, Peking University People's Hospital, Beijing, China.
Taiwan J Obstet Gynecol. 2015 Jun;54(3):284-9. doi: 10.1016/j.tjog.2015.01.002.
The aim of this study was to create a database of anatomical ultrathin cross-sectional images of fetal hearts with different congenital heart diseases (CHDs) and preliminarily to investigate its clinical application.
Forty Chinese fetal heart samples from induced labor due to different CHDs were cut transversely at 60-μm thickness. All thoracic organs were removed from the thoracic cavity after formalin fixation, embedded in optimum cutting temperature compound, and then frozen at -25°C for 2 hours. Subsequently, macro shots of the frozen serial sections were obtained using a digital camera in order to build a database of anatomical ultrathin cross-sectional images.
Images in the database clearly displayed the fetal heart structures. After importing the images into three-dimensional software, the following functions could be realized: (1) based on the original database of transverse sections, databases of sagittal and coronal sections could be constructed; and (2) the original and constructed databases could be displayed continuously and dynamically, and rotated in arbitrary angles. They could also be displayed synchronically. The aforementioned functions of the database allowed for the retrieval of images and three-dimensional anatomy characteristics of the different fetal CHDs, and virtualization of fetal echocardiography findings.
A database of 40 different cross-sectional fetal CHDs was established. An extensive database library of fetal CHDs, from which sonographers and students can study the anatomical features of fetal CHDs and virtualize fetal echocardiography findings via either centralized training or distance education, can be established in the future by accumulating further cases.
本研究旨在创建一个包含不同先天性心脏病（CHD）胎儿心脏的解剖学超薄横断面图像数据库，并初步探讨其临床应用。
收集40例因不同CHD引产的中国胎儿心脏样本，横向切成60μm厚。福尔马林固定后，将所有胸腔器官从胸腔中取出，嵌入最佳切割温度复合物中，然后在-25°C下冷冻2小时。随后，使用数码相机获取冷冻连续切片的宏观图像，以建立解剖学超薄横断面图像数据库。
数据库中的图像清晰显示了胎儿心脏结构。将图像导入三维软件后，可实现以下功能：（1）基于原始横断面数据库构建矢状面和冠状面数据库；（2）原始数据库和构建的数据库可连续动态显示，并可任意角度旋转，还可同步显示。数据库的上述功能允许检索不同胎儿CHD的图像和三维解剖特征，并实现胎儿超声心动图结果的虚拟化。
建立了一个包含40种不同横断面胎儿CHD的数据库。通过积累更多病例，未来可以建立一个广泛的胎儿CHD数据库库，超声检查人员和学生可以通过集中培训或远程教育从中研究胎儿CHD的解剖特征并实现胎儿超声心动图结果的虚拟化。
