Yguerabide J, Yguerabide E E
Department of Biology, University of California at San Diego, La Jolla, California, 92093, USA.
Anal Biochem. 1998 Sep 10;262(2):157-76. doi: 10.1006/abio.1998.2760.
In a companion article we present the idea that submicroscopic light-scattering particles, such as gold and silver particles, can be used as fluorescent analog tracers in biological and clinical applications. The light-emitting power and scattered light color of the particles can be adjusted by changing particle composition or diameter. Using Rayleigh and Mie light-scattering theory, we calculated the absorbance and light-scattering properties and power of particles of different diameters and selected compositions and compared them to the properties of fluorophores. In the present article, we evaluate experimentally the optical properties of particles of selected compositions and sizes and compare the results with the theoretically calculated values. We also discuss the methods which we use to measure the light-scattering properties of particles in suspension and to view individual particles by light microscopy. We also outline examples which demonstrate the use of light-scattering particles as fluorescent analogs in biological and clinical applications.
在一篇配套文章中，我们提出了这样的观点：亚微观光散射粒子，如金和银粒子，可在生物和临床应用中用作荧光类似物示踪剂。通过改变粒子组成或直径，可以调节粒子的发光能力和散射光颜色。利用瑞利和米氏光散射理论，我们计算了不同直径和选定组成的粒子的吸光度、光散射特性和能力，并将它们与荧光团的特性进行了比较。在本文中，我们通过实验评估了选定组成和尺寸的粒子的光学特性，并将结果与理论计算值进行了比较。我们还讨论了用于测量悬浮液中粒子的光散射特性以及通过光学显微镜观察单个粒子的方法。我们还概述了一些示例，这些示例展示了光散射粒子在生物和临床应用中作为荧光类似物的用途。
