一种用于硒醇的近红外荧光开启型探针的构建及其在活体动物中的生物成像应用
Construction of a Near-Infrared Fluorescent Turn-On Probe for Selenol and Its Bioimaging Application in Living Animals.
作者信息
Chen Hua, Dong Baoli, Tang Yonghe, Lin Weiying
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082 (P. R. China).
Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science of Jinan, Jinan, Shandong 250022 (P.R. China).
出版信息
Chemistry. 2015 Aug 10;21(33):11696-700. doi: 10.1002/chem.201502226. Epub 2015 Jul 14.
As selenocysteine (Sec) carries out the majority of the functions of the various Se-containing species in vivo, it is of high importance to develop reliable and rapid assays with biocompatibility to detect Sec. Herein, an NIR fluorescent turn-on probe for highly selective detection of selenol was designed and synthesized. The probe exhibits large turn-on signal upon treatment with selenocysteine (R-SeH), and it was further demonstrated that the new NIR fluorescent probe can be employed to image selenol in living animals.
由于硒代半胱氨酸（Sec）在体内执行各种含硒物种的大部分功能，因此开发具有生物相容性的可靠且快速的检测Sec的方法非常重要。在此，设计并合成了一种用于高选择性检测硒醇的近红外荧光开启探针。该探针对硒代半胱氨酸（R-SeH）处理表现出大的开启信号，并且进一步证明了这种新型近红外荧光探针可用于活体动物中硒醇的成像。
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