一种用于活细胞线粒体内SO衍生物成像的实时比率荧光探针。
A real-time ratiometric fluorescent probe for imaging of SO derivatives in mitochondria of living cells.
作者信息
Shi Junwei, Shu Wei, Tian Yong, Wu Yulong, Jing Jing, Zhang Rubo, Zhang Xiaoling
机构信息
Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology Beijing 100081 P. R. China
出版信息
RSC Adv. 2019 Jul 18;9(39):22348-22354. doi: 10.1039/c9ra03207j. eCollection 2019 Jul 17.
A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition that occurs between IN-CZ and sulfite with a detection limit of 2.99 × 10 M. IN-CZ displays a fast response (within 1 minute) and is highly selective for SO /HSO over ROS, biologically relevant ions, biological mercaptans and other reactive species. More importantly, IN-CZ was suitable for ratiometric fluorescence imaging in living cells, by real-time monitoring of SO /HSO changes in mitochondria targeted in living cells.
设计并合成了一种用于高选择性检测亚硫酸盐的实时比率荧光探针（IN-CZ），其基于对半菁染料平台分子内电荷转移（ICT）的调控。该探针的作用机制主要是通过IN-CZ与亚硫酸盐之间发生的迈克尔加成反应，检测限为2.99×10⁻⁶ M。IN-CZ显示出快速响应（在1分钟内），并且对SO₃²⁻/HSO₃⁻ 相对于活性氧（ROS）、生物相关离子、生物硫醇和其他活性物种具有高度选择性。更重要的是，IN-CZ适用于活细胞中的比率荧光成像，通过实时监测活细胞中线粒体靶向的SO₃²⁻/HSO₃⁻ 变化。
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