College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Talanta. 2020 May 15;212:120583. doi: 10.1016/j.talanta.2019.120583. Epub 2019 Nov 20.
Selenocysteine (Sec) is a primary kind of reactive selenium species in cells, and its vital roles in physiological processes have been characterized. Therefore, the highly effective method for sensing Sec in metabolic processes and selenium-rich food must be developed. This study presents a new fluorescent probe, namely, GSH-NB@AuNPs, for highly selective detection of selenol based on the fluorescence quenching quality on the surface of gold nanoparticles (AuNPs). The probe consists of glutathione (GSH) and Nile blue (NB) moieties assembled on AuNPs. The probe exhibits excellent sensitivity and selectivity for Sec and is applied in imaging endogenous and exogenous Sec in living cells through confocal fluorescence microscopy. The proposed probe provides a promising and powerful method for detecting selenol in foodstuff (such as selenium-rich rice and tea) with the detection limit of 9.5 nM.
硒代半胱氨酸（Sec）是细胞中主要的反应性硒物种，其在生理过程中的重要作用已得到阐明。因此，必须开发用于代谢过程和富硒食品中检测 Sec 的高效方法。本研究提出了一种新的荧光探针，即 GSH-NB@AuNPs，它基于金纳米粒子（AuNPs）表面的荧光猝灭质量，对硒醇具有高度选择性检测。探针由谷胱甘肽（GSH）和尼罗蓝（NB）部分组装在 AuNPs 上。该探针对 Sec 具有优异的灵敏度和选择性，并通过共聚焦荧光显微镜应用于活细胞内内源性和外源性 Sec 的成像。该探针为检测食品（如富硒大米和茶）中的硒醇提供了一种有前途和强大的方法，检测限为 9.5 nM。
