Kong Fanpeng, Ge Lihong, Pan Xiaohong, Xu Kehua, Liu Xiaojun, Tang Bo
College of Chemistry , Chemical Engineering and Materials Science , Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong , Key Laboratory of Molecular and Nano Probes , Ministry of Education , Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals , Shandong Normal University , Jinan 250014 , P. R. China . Email:
Chem Sci. 2016 Feb 1;7(2):1051-1056. doi: 10.1039/c5sc03471j. Epub 2015 Oct 28.
Hydrogen selenide (HSe), a highly reactive Se species, is an important selenium metabolism intermediate involved in many physiological and pathological processes. This compound is of scientific interest with regard to the real-time monitoring of HSe in living cells and to understand the anti-cancer mechanism of selenium. However, monitoring HSe in living cells is still challenging due to the lack of straight forward, highly selective and rapid methods. Here, we developed a novel small-molecule fluorescent probe, , for imaging endogenous HSe. exhibited high selectivity toward HSe over selenocysteine (Sec), HS and small molecule thiols and was successfully used to image the HSe content in HepG2 cells during NaSeO-induced apoptosis. Increased HSe content and reduced ROS levels were observed under hypoxic conditions compared to normoxic conditions, which indicated that the cell apoptosis induced by NaSeO under a hypoxic environment is a non-oxidative stress mechanism. Thus, this probe should serve as a powerful tool for exploring the physiological function of HSe and Se anticancer mechanisms in a variety of physiological and pathological contexts.
硒化氢（HSe）是一种高反应性的硒物种，是参与许多生理和病理过程的重要硒代谢中间体。就实时监测活细胞中的HSe以及了解硒的抗癌机制而言，这种化合物具有科学研究价值。然而，由于缺乏直接、高选择性和快速的方法，在活细胞中监测HSe仍然具有挑战性。在此，我们开发了一种新型小分子荧光探针，用于对内源性HSe进行成像。该探针对HSe的选择性高于硒代半胱氨酸（Sec）、HS和小分子硫醇，并成功用于在亚硒酸钠诱导的细胞凋亡过程中对HepG2细胞中的HSe含量进行成像。与常氧条件相比，在低氧条件下观察到HSe含量增加和ROS水平降低，这表明在低氧环境下亚硒酸钠诱导的细胞凋亡是一种非氧化应激机制。因此，该探针应成为在各种生理和病理背景下探索HSe的生理功能和硒抗癌机制的有力工具。
