Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Bioprocess Biosyst Eng. 2013 Mar;36(3):365-73. doi: 10.1007/s00449-012-0793-2. Epub 2012 Aug 4.
The production of laccase by immobilized mycelia of Peniophora cinerea and Trametes versicolor was studied. In an initial stage, experimental assays were performed in Erlenmeyer flasks using free and immobilized mycelium, and the performance of the fungal strains to produce the enzyme was compared. Both fungi adhered into the support material (a synthetic fiber), growing not only on the surface but also in the interspaces of the fibers. Immobilization of P. cinerea provided a 35-fold increase in laccase production when compared to the production obtained by using free mycelium. On the other hand, immobilization of T. versicolor caused a decrease in laccase activity. A comparison between the strains revealed that immobilized P. cinerea (3,500 U/L) surpassed the enzyme production by free T. versicolor (800 U/L). When the conditions that gave the best laccase production to each fungus were employed in a stirred tank bioreactor, very low laccase production was observed for both the cases, suggesting that shear stress and mycelia damage caused by the agitation impellers negatively affected the enzyme production.
研究了栓菌和彩绒革盖菌固定化菌丝体产漆酶的情况。在初步阶段，使用游离和固定化菌丝体在 Erlenmeyer 摇瓶中进行了实验，比较了真菌菌株产生酶的性能。两种真菌都附着在支撑材料（一种合成纤维）上，不仅在纤维表面生长，而且在纤维间隙中生长。与使用游离菌丝体相比，固定化栓菌可使漆酶产量增加 35 倍。另一方面，固定化彩绒革盖菌会降低漆酶活性。对两种菌株的比较表明，固定化栓菌（3500 U/L）超过了游离彩绒革盖菌（800 U/L）的酶产量。当在搅拌罐生物反应器中采用最适合每种真菌产漆酶的条件时，两种情况下的漆酶产量都非常低，这表明搅拌器的剪切力和菌丝体损伤会对酶的产生产生负面影响。
