一种用于测定马补体的溶血试验。

A hemolytic assay for the measurement of equine complement.

作者信息

Reis K J

机构信息

Department of Large Animal Clinical Sciences, University of Florida, Gainesville 32610.

出版信息

Vet Immunol Immunopathol. 1989 Nov 30;23(1-2):129-37. doi: 10.1016/0165-2427(89)90115-3.

DOI:10.1016/0165-2427(89)90115-3

PMID:2617845

Abstract

A hemolytic assay was developed for the measurement of functional equine complement activity. The assay utilizes antibody sensitized chicken erythrocytes as the target cell and was specific for classical pathway (antibody dependent) complement activity. The assay was found to be reproducible and more sensitive than previous reports using other species of target cells. Total serum complement (CH50) values were determined for five mares and their foals and followed over a period of 3 months.

摘要

开发了一种用于测量马功能性补体活性的溶血试验。该试验利用抗体致敏的鸡红细胞作为靶细胞，对经典途径（抗体依赖性）补体活性具有特异性。发现该试验具有可重复性，并且比以前使用其他种类靶细胞的报告更敏感。测定了5匹母马及其驹的血清总补体（CH50）值，并在3个月的时间内进行跟踪。