三种不同方法测量经典途径补体活性的比较。
Comparison of three different methods for measuring classical pathway complement activity.
作者信息
Jaskowski T D, Martins T B, Litwin C M, Hill H R
机构信息
Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Pediatrics and Medicine, University of Utah School of Medicine, Salt Lake City, Utah 84108, USA.
出版信息
Clin Diagn Lab Immunol. 1999 Jan;6(1):137-9. doi: 10.1128/CDLI.6.1.137-139.1999.
Abstract
The complement system plays an important role in host defense against infection and in most inflammatory processes. The standard 50% hemolytic complement (CH50) assay is the most commonly used method of screening patient sera for functional activity of the classical complement pathway. Our objective in this study was to compare two newer methods (the enzyme immunoassay and the liposome immunoassay) to a commercial CH50 assay for measuring total classical complement activity. We conclude that both newer methods compare well with a CH50 assay and are equally sensitive in screening routine clinical sera.
摘要
补体系统在宿主抗感染防御及大多数炎症过程中发挥着重要作用。标准的50%溶血补体（CH50）检测是筛查患者血清中经典补体途径功能活性最常用的方法。我们在本研究中的目的是将两种较新的方法（酶免疫测定法和脂质体免疫测定法）与一种商用CH50检测法进行比较，以测定总经典补体活性。我们得出结论，这两种较新的方法与CH50检测法相比效果良好，且在筛查常规临床血清时同样敏感。
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本文引用的文献
1
The development of the complement system after 28 weeks' gestation.妊娠28周后补体系统的发育。
Acta Paediatr. 1997 May;86(5):523-7. doi: 10.1111/j.1651-2227.1997.tb08924.x.
2
The Roche Rheumatology Prize Lecture. Complement deficiency and disease.罗氏风湿病学奖讲座。补体缺陷与疾病。
Br J Rheumatol. 1993 Apr;32(4):269-73. doi: 10.1093/rheumatology/32.4.269.
3
Some aspects of the humoral immunity and the phagocytic function in newborn infants.新生儿体液免疫和吞噬功能的某些方面。
Isr J Med Sci. 1994 May-Jun;30(5-6):331-5.
4
[Development of the serum levels of complement during the first year of life].[生命第一年血清补体水平的发育]
An Esp Pediatr. 1980 Jul;13(7):571-6.
5
The complement system of the newborn infant.新生儿的补体系统。
Biol Neonate. 1980;37(3-4):209-17. doi: 10.1159/000241276.
6
Inherited complement component abnormalities.遗传性补体成分异常。
Annu Rev Med. 1986;37:333-46. doi: 10.1146/annurev.me.37.020186.002001.
7
Quantitation of the anaphylatoxin C3a in the presence of C3 by a novel sandwich ELISA using monoclonal antibody to a C3a neoepitope.利用针对C3a新表位的单克隆抗体，通过新型夹心酶联免疫吸附测定法在存在C3的情况下对过敏毒素C3a进行定量分析。
J Immunol Methods. 1989 Jul 26;121(2):261-8. doi: 10.1016/0022-1759(89)90169-5.
8
Quantification of the terminal complement complex in human plasma by an enzyme-linked immunosorbent assay based on monoclonal antibodies against a neoantigen of the complex.基于针对补体终末复合物新抗原的单克隆抗体，采用酶联免疫吸附测定法对人血浆中的补体终末复合物进行定量分析。
Scand J Immunol. 1985 Aug;22(2):197-202. doi: 10.1111/j.1365-3083.1985.tb01871.x.
9
Complement factor 9 deficiency in serum of human neonates.人类新生儿血清中补体因子9缺乏
J Infect Dis. 1992 Jul;166(1):53-7. doi: 10.1093/infdis/166.1.53.
10
Complement deficiencies.补体缺陷
Annu Rev Immunol. 1992;10:809-34. doi: 10.1146/annurev.iy.10.040192.004113.
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