一种快速简便的鸡溶血补体检测技术。
A rapid and simplified technique for the assay of chicken hemolytic complement.
作者信息
Skeeles J K, Lukert P D, DeBuysscher E V
出版信息
Am J Vet Res. 1979 Mar;40(3):443-5.
The technique described is a modification of a qualitative hemolytic radial diffusion technique. The test involves the use of sensitized sheep erythrocytes that have been incorporated into agarose. Tube dilutions were made of chicken serum and samples of each dilution were placed into wells cut in the agarose. The test is quantitative for hemolytic complement in that the highest dilution showing visible hemolysis of sensitized erythrocytes in agarose is determined to be the endpoint for that serum sample. The test as compared with the standard tube assay was determined to be less sensitive by approximately one dilution. The advantages of speed, simplicity, and cost more than offset the decrease in sensitivity of the test.
所描述的技术是对定性溶血径向扩散技术的一种改进。该测试涉及使用已掺入琼脂糖中的致敏绵羊红细胞。对鸡血清进行试管稀释，并将每种稀释液的样品放入琼脂糖中切割出的孔中。该测试对溶血补体是定量的，因为在琼脂糖中显示致敏红细胞可见溶血的最高稀释度被确定为该血清样品的终点。与标准试管试验相比，该测试的灵敏度约低一个稀释度。速度快、操作简单和成本低等优点足以弥补测试灵敏度的降低。
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