Mengoni Tamara, Peregrina Dolores Vargas, Censi Roberta, Cortese Manuela, Ricciutelli Massimo, Maggi Filippo, Di Martino Piera
a School of Pharmacy, University of Camerino , Camerino , Italy.
b HPLC-MS laboratory, University of Camerino , Camerino , Italy.
Nat Prod Res. 2016;30(3):268-75. doi: 10.1080/14786419.2015.1055491. Epub 2015 Jul 16.
The aim of this work was to provide a characterisation of volatile constituents from different commercial batches of henna (Lawsonia inermis) leaves of different geographic origin. Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) was used for the purpose. A total of 72 components were identified by GC-MS in the headspace of different henna samples which proved to differ considerably from each other, because they were characterised by different classes of components, mainly aliphatic compounds (9.0-64.7%), terpenoids (5.8-45.5%) and aromatics (7.9-45.2%), with alkanes (0.9-18.5%), aldehydes (2.1-18.8%) and carboxylic acids (3.1-29.3%), monoterpenes (3.4-30.0%) and sesquiterpenes (0.8-23.7%) and phenyl propanoids (0.6-43.1%), being the most abundant, respectively. Major representatives of these groups were n-hexadecane (0.5-4.7%), (2E)-hexenal (0.5-11.7%) and acetic acid (2.8-24.5%), limonene (0.8-14.7%), carvol (3.8-7.1%), geranyl acetone (1.4-7.9%) and (E)-caryophyllene (3.3-8.4%), and (E)-anethole (0.6-35.0%), respectively. We assume that factors such as the manufacturing process, the storage conditions and the different geographic origin of the samples may contribute to such variability.
这项工作的目的是对不同地理来源的不同商业批次指甲花叶（散沫花）中的挥发性成分进行表征。为此采用了顶空固相微萃取（HS-SPME）结合气相色谱-质谱联用（GC-MS）的方法。通过GC-MS在不同指甲花样品的顶空中共鉴定出72种成分，这些成分彼此间差异很大，因为它们具有不同类别的成分，主要是脂肪族化合物（9.0 - 64.7%）、萜类化合物（5.8 - 45.5%）和芳香族化合物（7.9 - 45.2%），其中烷烃（0.9 - 18.5%）、醛类（2.1 - 18.8%）和羧酸（3.1 - 29.3%）、单萜（3.4 - 30.0%）和倍半萜（0.8 - 23.7%）以及苯丙烷类（0.6 - 43.1%）分别最为丰富。这些类别的主要代表分别是正十六烷（0.5 - 4.7%）、（2E）-己烯醛（0.5 - 11.7%）和乙酸（2.8 - 24.5%）、柠檬烯（0.8 - 14.7%）、香芹酚（3.8 - 7.1%）、香叶基丙酮（1.4 - 7.9%）和（E）-石竹烯（3.3 - 8.4%）以及（E）-茴香脑（0.6 - 35.0%）。我们认为，诸如制造工艺、储存条件以及样品的不同地理来源等因素可能导致了这种变异性。
