Department of Food Biotechnology and Science, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos, 09001 Burgos, Spain.
J Agric Food Chem. 2015 Aug 12;63(31):6922-31. doi: 10.1021/acs.jafc.5b01644. Epub 2015 Jul 30.
Evaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and highlight the validity of QUENCHER (Q) methods, values of Q-method validation were showed for the first time, and they were tested with products of well-known different chemical properties. Furthermore, new QUENCHER assays to measure scavenging capacity against superoxide, hydroxyl, and lipid peroxyl radicals were developed. Calibration models showed good linearity (R(2) > 0.995), proportionality and precision (CV < 6.5%), and acceptable detection limits (<20.4 nmol Trolox equiv). The presence of ethanol in the reaction medium gave antioxidant capacity values significantly different from those obtained with water. The dilution of samples with powdered cellulose was discouraged because possible interferences with some of the matrices analyzed may take place.
无需提取步骤即可评估固体基质的总抗氧化能力，这对于食品研究人员和食品行业来说是一个非常有趣的选择。这些方法被称为 QUENCHER，是 QUick、Easy、New、CHEap 和 Reproducible 测定法的缩写。为了证明和强调 QUENCHER (Q) 方法的有效性，首次展示了 Q 方法验证的值，并对具有不同化学性质的知名产品进行了测试。此外，还开发了新的 QUENCHER 测定法来测量对超氧阴离子、羟自由基和脂质过氧自由基的清除能力。校准模型显示出良好的线性（R(2) > 0.995）、比例性和精密度（CV < 6.5%），以及可接受的检测限（<20.4 nmol Trolox equiv）。反应介质中存在乙醇会导致抗氧化能力值与用水获得的值显著不同。不鼓励用粉状纤维素稀释样品，因为可能会与某些分析的基质发生干扰。
