Kurnik Katarzyna, Treder Krzysztof, Skorupa-Kłaput Monika, Tretyn Andrzej, Tyburski Jarosław
Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, Poland.
Laboratory of Molecular Diagnostic and Biochemistry, Department of Potato Protection and Seed Science in Bonin, Plant Breeding and Acclimatization Institute-National Research Institute, 76-009 Bonin, Poland.
Water Air Soil Pollut. 2015;226(8):254. doi: 10.1007/s11270-015-2517-0. Epub 2015 Jul 11.
Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains large amounts of active peroxidases. We demonstrate that potato pulp may serve as a tool for peroxidase-based remediation of phenol pollution. The phenol removal efficiency of potato pulp was over 95 % for optimized phenol concentrations. The potato pulp enzymes maintained their activity at pH 4 to 8 and were stable over a wide temperature range. Phenol solutions treated with potato pulp showed a significant reduction in toxicity compared with untreated phenol solutions. Finally we determined that this method may be employed to remove phenol from industrial effluent with over 90 % removal efficiency under optimal conditions.
植物过氧化物酶在苯酚污染废水的净化方面具有强大的潜在应用价值。然而，大规模使用这些酶进行苯酚脱污需要一种廉价、丰富且易于获取的含过氧化物酶材料来源。在本研究中，我们表明淀粉工业的废弃物马铃薯浆含有大量活性过氧化物酶。我们证明马铃薯浆可作为基于过氧化物酶修复苯酚污染的工具。对于优化后的苯酚浓度，马铃薯浆的苯酚去除效率超过95%。马铃薯浆中的酶在pH值4至8时保持活性，并且在很宽的温度范围内都很稳定。与未处理的苯酚溶液相比，用马铃薯浆处理的苯酚溶液毒性显著降低。最后我们确定，在最佳条件下，该方法可用于从工业废水中去除苯酚，去除效率超过90%。
