Gysbrechts Barbara, Wang Ling, Trong Nghia Nguyen Do, Cabral Henrique, Navratilova Zaneta, Battaglia Francesco, Saeys Wouter, Bartic Carmen
Department of Physics and Astronomy, University of Leuven, Celestijnenlaan 200D, 3001, Leuven, Belgium.
Department of Biosystems, University of Leuven, Kasteelpark Arenberg 30, 3001, Leuven, Belgium.
J Biophotonics. 2016 Jun;9(6):576-85. doi: 10.1002/jbio.201500106. Epub 2015 Jul 20.
Optical brain stimulation gained a lot of attention in neuroscience due to its superior cell-type specificity. In the design of illumination strategies, predicting the light propagation in a specific tissue is essential and requires knowledge of the optical properties of that tissue. We present the estimated absorption and reduced scattering in rodent brain tissue using non-destructive contact spatially resolved spectroscopy (cSRS). The obtained absorption and scattering in the cortex, hippocampus and striatum are similar, but lower than in the thalamus, leading to a less deep but broader light penetration profile in the thalamus. Next, the light distribution was investigated for different stimulation protocols relevant for fiber-optic based optogenetic experiments, using Monte Carlo simulation. A protocol specific analysis is proposed to evaluate the potential of thermally induced side effects.
由于其卓越的细胞类型特异性，光学脑刺激在神经科学领域备受关注。在照明策略的设计中，预测特定组织中的光传播至关重要，这需要了解该组织的光学特性。我们使用非破坏性接触空间分辨光谱学（cSRS）展示了啮齿动物脑组织中的估计吸收和约化散射。在皮质、海马体和纹状体中获得的吸收和散射相似，但低于丘脑，导致丘脑的光穿透深度较浅但范围更广。接下来，使用蒙特卡罗模拟研究了与基于光纤的光遗传学实验相关的不同刺激方案的光分布。提出了一种针对方案的分析方法来评估热诱导副作用的可能性。
