Gajdosová E, Mayer V
Acta Virol. 1978 Mar;22(2):131-8.
In a long-term experiment, the features of humoral and cell-mediated immunity (CMI) were investigated in mice given intravenously (i.v.) a single dose of live attenuated Langat (E5 "14") virus of the tick-borne encephalitis (TBE) complex. The inapparent infection induced in mice specific resistance against challenge with virulent TBE virus, CMI was assayed by the capillary method of murine splenic lymphocyte migrarion inhibition (LMI) in the presence of partially purified TBE vurus. Significant LMI values were observed as early as 4 days after inoculation (p.i.), persisting for at least 15 months. Specific virus neutralizing (VN) antibodies were detected in the pooled serum of mice on the 4th day p.i. by a VN test potentiated with monospecific anti-mouse IgG serum. For the whole experimental period, significant antibody levels were found in the immunized mice which also displayed a complete resistance against 1000 s.c. LD50 of the virulent virus. Organ cultures of brain and spleen from these mice did not yield infections virus.
在一项长期实验中，对静脉注射（i.v.）单剂量蜱传脑炎（TBE）复合体减毒活朗加特（E5 “14”）病毒的小鼠的体液免疫和细胞介导免疫（CMI）特征进行了研究。小鼠体内诱发的隐性感染使其对强毒TBE病毒攻击产生特异性抵抗力，采用毛细管法检测在部分纯化的TBE病毒存在下小鼠脾淋巴细胞迁移抑制（LMI）来测定CMI。接种后（p.i.）4天就观察到显著的LMI值，且持续至少15个月。通过用单特异性抗小鼠IgG血清增强的病毒中和（VN）试验，在接种后第4天检测到小鼠混合血清中的特异性病毒中和（VN）抗体。在整个实验期间，免疫小鼠体内发现显著的抗体水平，这些小鼠对1000个皮下半数致死剂量（s.c. LD50）的强毒病毒也表现出完全抵抗力。这些小鼠的脑和脾器官培养物未产生感染性病毒。
