Mayer V, Pogády J, Starek M, Hrbka J
Acta Virol. 1975 May;19(3):229-36.
The clinical and immunological events in volunteers following administration of 5 and 6 dex newborn mouse intracerebral LD5O (NmicLD5O) of the E5 "14" virus clone, segregated from the Langat TP21 E5 strain (tick-borne encephalitis-TE-complex) and propagated in 7 days old SPF chick embryos, are reported. The experimental vaccine, containing the cloned virus, carrying a set of genetic markers of an ic(+) sc s(+/-) t e u (s) N character, caused in volunteers no clinically recognizable effects, but elicited an immune response in them, as determined in PS-cell culture neutralization tests with various TE (western and eastern subtype) strains. In all vaccines, given the virus intramuscularly, seroconversions from negativity to positivity were observed, with antibody titres ranging from 4-128. The parenteral virus administration was superior to the oral route.
报道了从Langat TP21 E5株（蜱传脑炎-TE-复合体）分离并在7日龄SPF鸡胚中传代的E5“14”病毒克隆，给志愿者接种5和6倍新生小鼠脑内半数致死剂量（NmicLD50）后志愿者的临床和免疫事件。含有克隆病毒的实验疫苗带有一组ic(+) sc s(+/-) t e u (s) N特征的遗传标记，在志愿者中未引起临床可识别的效应，但在用各种TE（西方和东方亚型）毒株进行的PS细胞培养中和试验中引发了他们的免疫反应。在所有通过肌肉注射给予病毒的疫苗接种中，观察到了从阴性到阳性的血清学转换，抗体滴度范围为4 - 128。肠胃外给予病毒优于口服途径。
