不同成熟阶段的海棠果中红厚壳内酯的含量及其成骨活性。
Calophyllolide content in Calophyllum inophyllum at different stages of maturity and its osteogenic activity.
作者信息
Liu Wei-Hsien, Liu Yen-Wenn, Chen Zih-Fong, Chiou Wen-Fei, Tsai Ying-Chieh, Chen Chien-Chih
机构信息
Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, Beitou Dist., Taipei 11221, Taiwan.
National Research Institute of Chinese Medicine, No. 155-1, Li-Nong St., Sec. 2, Beitou Dist., Taipei 11221, Taiwan.
出版信息
Molecules. 2015 Jul 7;20(7):12314-27. doi: 10.3390/molecules200712314.
Calophyllum inophyllum is a coastal plant rich in natural substances. Its ingredients have been used for the development of an anti-human immunodeficiency virus (HIV) drug. In this study, we collected C. inophyllum fruit, and the ethanol extract of the fruit was chromatographically separated using silica gel and Sephadex LH-20 columns to obtain the major compound, calophyllolide. The fruits were harvested from September to December in 2011; a quantitative analysis of the calophyllolide content was conducted using HPLC to explore the differences between the different parts of the fruit during the growing season. The results showed that in fruits of C. inophyllum, calophyllolide exists only in the nuts, and dried nuts contain approximately 2 mg·g-1 of calophyllolide. The calophyllolide levels in the nuts decreased during maturity. In addition, calophyllolide dose-dependently enhanced alkaline phosphatase (ALP) activity in murine osteoblastic MC3T3-E1 cells, without significant cytotoxicity. The expression of osteoblastic genes, ALP and osteocalcin (OCN), were increased by calophyllolide. Calophyllolide induced osteoblasts differentiation also evidenced by increasing mineralization and ALP staining.
红厚壳是一种富含天然物质的沿海植物。其成分已被用于开发一种抗人类免疫缺陷病毒（HIV）药物。在本研究中，我们采集了红厚壳果实，果实的乙醇提取物通过硅胶柱和葡聚糖凝胶LH - 20柱进行色谱分离，得到主要化合物红厚壳内酯。果实于2011年9月至12月收获；采用高效液相色谱法对红厚壳内酯含量进行定量分析，以探讨果实不同部位在生长季节的差异。结果表明，在红厚壳果实中，红厚壳内酯仅存在于果仁中，干果仁中红厚壳内酯含量约为2 mg·g-1。果仁中红厚壳内酯水平在成熟过程中降低。此外，红厚壳内酯剂量依赖性地增强了小鼠成骨细胞MC3T3 - E1细胞中的碱性磷酸酶（ALP）活性，且无明显细胞毒性。红厚壳内酯增加了成骨细胞基因ALP和骨钙素（OCN）的表达。红厚壳内酯诱导成骨细胞分化还通过矿化增加和ALP染色得到证实。
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