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对疑似医院感染败血症新生儿进行Light-Cycler® SeptiFast检测的评估。

Evaluation of the Light-Cycler® SeptiFast Test in Newborns With Suspicion of Nosocomial Sepsis.

作者信息

Ortiz Ibarra Javier, Trevino Valdez Pablo, Valenzuela Mendez Ema, Limon Rojas Ana, Lara Flores Gabriel, Ceballos Bocanegra Adrian, Morales Mendez Iyari, Fernandez Carrocera Luis, Covian Molina Emilia, Reyna Figueroa Jesus

机构信息

Research Department, Diagnomol Laboratories, Mexico City Mexico.

Infectious Diseases Department, University Hospital, Monterrey Nuevo Leon, Mexico.

出版信息

Iran J Pediatr. 2015 Feb;25(1):e253. doi: 10.5812/ijp.253. Epub 2015 Jan 17.

DOI:10.5812/ijp.253
PMID:26199693
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4505975/
Abstract

BACKGROUND

Nosocomial sepsis (NS) in newborns (NBs) is associated with high mortality rates and low microbial recovery rates. To overcome the latter problem, new techniques in molecular biology are being used.

OBJECTIVES

To evaluate the diagnostic efficacy of SeptiFast test for the diagnosis of nosocomial sepsis in the newborn.

MATERIALS AND METHODS

86 blood specimens of NBs with suspected NS (NOSEP-1 Test > 8 points) were analyzed using Light Cycler SeptiFast (LC-SF) a real-time multiplex PCR instrument. The results were analyzed with the Roche SeptiFast Identification Software. Another blood sample was collected to carry out a blood culture (BC).

RESULTS

Sensitivity (Sn) and specificity (Sp) of 0.69 and 0.65 respectively, compared with blood culture (BC) were obtained for LC-SF. Kappa index concordance between LC-SF and BC was 0.21. Thirteen (15.11%) samples were BC positive and 34 (31.39%) were positive with LC-SF tests.

CONCLUSIONS

Compared with BC, LC-SF allows the detection of a greater number of pathogenic species in a small blood sample (1 mL) with a shorter response time.

摘要

背景

新生儿医院感染性败血症（NS）与高死亡率和低微生物检出率相关。为克服后一问题，分子生物学新技术正被应用。

目的

评估SeptiFast检测对新生儿医院感染性败血症的诊断效能。

材料与方法

使用实时多重聚合酶链反应仪器Light Cycler SeptiFast（LC-SF）分析86例疑似NS（NOSEP-1检测>8分）新生儿的血标本。结果用罗氏SeptiFast鉴定软件分析。采集另一血标本进行血培养（BC）。

结果

与血培养相比，LC-SF的灵敏度（Sn）和特异度（Sp）分别为0.69和0.65。LC-SF与BC之间的Kappa指数一致性为0.21。13份（15.11%）样本血培养阳性，34份（31.39%）样本LC-SF检测阳性。

结论

与血培养相比，LC-SF能在少量血标本（1 mL）中检测出更多病原菌，且响应时间更短。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db06/4505975/e5b61bb9f78c/ijp-25-253-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db06/4505975/1bfb6c33f1d9/ijp-25-253-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db06/4505975/e5b61bb9f78c/ijp-25-253-i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db06/4505975/1bfb6c33f1d9/ijp-25-253-i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db06/4505975/e5b61bb9f78c/ijp-25-253-i002.jpg

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Intra-abdominal spp infection in acute abdomen in a quality assurance (QA)-certified academic setting.在一家获得质量保证（QA）认证的学术机构中，急性腹痛患者的腹腔内特定病原体感染情况。
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