• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay.

作者信息

Janetzki Sylvia

机构信息

ZellNet Consulting, Inc., 555 North Avenue, Ste. 25-S, Fort Lee, NJ 07024 USA.

出版信息

J Immunother Cancer. 2015 Jul 21;3:30. doi: 10.1186/s40425-015-0074-0. eCollection 2015.

DOI:10.1186/s40425-015-0074-0
PMID:26199727
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4508819/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99d1/4508819/b429004e0eb4/40425_2015_74_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99d1/4508819/b429004e0eb4/40425_2015_74_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99d1/4508819/b429004e0eb4/40425_2015_74_Fig1_HTML.jpg

相似文献

1
Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay.免疫监测技术入门：酶联免疫斑点法（Elispot）和荧光斑点检测法。
J Immunother Cancer. 2015 Jul 21;3:30. doi: 10.1186/s40425-015-0074-0. eCollection 2015.
2
Detection and Enumeration of Cytokine-Secreting Cells by FluoroSpot.利用 FluoroSpot 检测和计数细胞因子分泌细胞。
Methods Mol Biol. 2022;2386:81-99. doi: 10.1007/978-1-0716-1771-7_6.
3
Multi-Color FLUOROSPOT Counting Using ImmunoSpot Fluoro-X™ Suite.使用免疫斑点荧光X™套件进行多色荧光斑点计数
Methods Mol Biol. 2018;1808:115-131. doi: 10.1007/978-1-4939-8567-8_10.
4
Enzyme-Linked ImmunoSpot (ELISpot) for Single-Cell Analysis.用于单细胞分析的酶联免疫斑点法（ELISpot）
Methods Mol Biol. 2015;1346:27-46. doi: 10.1007/978-1-4939-2987-0_3.
5
Fluorospot assay: methodological analysis.荧光斑点试验：方法学分析。
Methods Mol Biol. 2005;302:289-96. doi: 10.1385/1-59259-903-6:289.
6
Dual- and triple-color fluorospot.双色和三色荧光斑点检测
Methods Mol Biol. 2012;792:77-85. doi: 10.1007/978-1-61779-325-7_6.
7
Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays.用于单色和双色ELISPOT及FLUOROSPOT检测的膜微孔板
Methods Mol Biol. 2015;1312:435-47. doi: 10.1007/978-1-4939-2694-7_44.
8
Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays.加强 ELISpot：荧光斑点分析中的多层次分析。
Cells. 2014 Nov 27;3(4):1102-15. doi: 10.3390/cells3041102.
9
Enzyme-linked immunospot assay response to recombinant CFP-10/ESAT-6 fusion protein among patients with spinal tuberculosis: implications for diagnosis and monitoring of surgical therapy.酶联免疫斑点法检测重组 CFP-10/ESAT-6 融合蛋白在脊柱结核患者中的反应：对手术治疗诊断和监测的意义。
Int J Infect Dis. 2013 Sep;17(9):e733-8. doi: 10.1016/j.ijid.2013.02.027. Epub 2013 Apr 25.
10
Combined antigen-specific interferon-γ and interleukin-2 release assay (FluoroSpot) for the diagnosis of Mycobacterium tuberculosis infection.联合抗原特异性干扰素-γ和白细胞介素-2释放试验（荧光斑点法）用于诊断结核分枝杆菌感染。
PLoS One. 2015 Mar 18;10(3):e0120006. doi: 10.1371/journal.pone.0120006. eCollection 2015.

引用本文的文献

1
IFN-γ/IL-2 Double-Color FluoroSpot Assay for Monitoring Human Primary T Cell Activation: Validation, Inter-Laboratory Comparison, and Recommendations for Clinical Studies.用于监测人原代T细胞活化的IFN-γ/IL-2双色荧光斑点试验：验证、实验室间比较及临床研究建议
AAPS J. 2025 Apr 25;27(4):81. doi: 10.1208/s12248-025-01072-3.
2
High-Plex ELISPOT: FOLISPOT Based on Fluorescence Detection and DNA Complementary Pairing.高通量 ELISPOT：基于荧光检测和 DNA 互补配对的 FOLISPOT。
Methods Mol Biol. 2024;2768:273-296. doi: 10.1007/978-1-0716-3690-9_16.
3
Clinical relevance of the combined analysis of circulating tumor cells and anti-tumor T-cell immunity in metastatic breast cancer patients.

本文引用的文献

1
Improvement of IFNg ELISPOT Performance Following Overnight Resting of Frozen PBMC Samples Confirmed Through Rigorous Statistical Analysis.通过严格的统计分析证实，冷冻PBMC样本过夜静置后IFNg ELISPOT性能得到改善。
Cells. 2014 Dec 24;4(1):1-18. doi: 10.3390/cells4010001.
2
Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays.加强 ELISpot：荧光斑点分析中的多层次分析。
Cells. 2014 Nov 27;3(4):1102-15. doi: 10.3390/cells3041102.
3
Sipuleucel-T immune parameters correlate with survival: an analysis of the randomized phase 3 clinical trials in men with castration-resistant prostate cancer.
转移性乳腺癌患者循环肿瘤细胞与抗肿瘤T细胞免疫联合分析的临床相关性
Front Oncol. 2022 Aug 23;12:983887. doi: 10.3389/fonc.2022.983887. eCollection 2022.
4
SITC cancer immunotherapy resource document: a compass in the land of biomarker discovery.SITC 癌症免疫治疗资源文件：生物标志物发现领域的指南针。
J Immunother Cancer. 2020 Dec;8(2). doi: 10.1136/jitc-2020-000705.
5
The Society for Immunotherapy of Cancer Biomarkers Task Force recommendations review.癌症免疫治疗生物标志物学会工作组建议审查。
Semin Cancer Biol. 2018 Oct;52(Pt 2):12-15. doi: 10.1016/j.semcancer.2017.09.006. Epub 2017 Sep 22.
6
Immunotherapy biomarkers 2016: overcoming the barriers.免疫疗法生物标志物 2016：突破障碍。
J Immunother Cancer. 2017 Mar 21;5(1):29. doi: 10.1186/s40425-017-0225-6.
7
Current status and perspectives in translational biomarker research for PD-1/PD-L1 immune checkpoint blockade therapy.PD-1/PD-L1免疫检查点阻断疗法的转化生物标志物研究现状与展望
J Hematol Oncol. 2016 May 27;9(1):47. doi: 10.1186/s13045-016-0277-y.
西普乐他赛免疫参数与生存相关：一项针对去势抵抗性前列腺癌男性的随机 3 期临床试验分析。
Cancer Immunol Immunother. 2013 Jan;62(1):137-47. doi: 10.1007/s00262-012-1317-2. Epub 2012 Aug 3.
4
Multipeptide immune response to cancer vaccine IMA901 after single-dose cyclophosphamide associates with longer patient survival.单次剂量环磷酰胺后，IMA901 癌症疫苗的多肽免疫反应与患者更长的生存时间相关。
Nat Med. 2012 Aug;18(8):1254-61. doi: 10.1038/nm.2883. Epub 2012 Jul 29.
5
The impact of harmonization on ELISPOT assay performance.协调对酶联免疫斑点试验性能的影响。
Methods Mol Biol. 2012;792:25-36. doi: 10.1007/978-1-61779-325-7_2.
6
Response definition criteria for ELISPOT assays revisited.ELISPOT 检测反应定义标准的再探讨。
Cancer Immunol Immunother. 2010 Oct;59(10):1489-501. doi: 10.1007/s00262-010-0875-4. Epub 2010 Jun 15.
7
Vaccination against HPV-16 oncoproteins for vulvar intraepithelial neoplasia.针对人乳头瘤病毒16型癌蛋白的疫苗用于外阴上皮内瘤变
N Engl J Med. 2009 Nov 5;361(19):1838-47. doi: 10.1056/NEJMoa0810097.
8
Detection of HIV vaccine-induced cell-mediated immunity in HIV-seronegative clinical trial participants using an optimized and validated enzyme-linked immunospot assay.使用优化且经过验证的酶联免疫斑点试验在HIV血清阴性的临床试验参与者中检测HIV疫苗诱导的细胞介导免疫。
J Acquir Immune Defic Syndr. 2007 May 1;45(1):20-7. doi: 10.1097/QAI.0b013e3180377b5b.
9
Sensitivity of a new commercial enzyme-linked immunospot assay (T SPOT-TB) for diagnosis of tuberculosis in clinical practice.一种新型商用酶联免疫斑点试验（T SPOT-TB）在临床实践中诊断结核病的敏感性。
Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):529-36. doi: 10.1007/s10096-005-1377-8.
10
A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells.一种用于计数特异性抗体分泌细胞的固相酶联免疫斑点（ELISPOT）检测法。
J Immunol Methods. 1983 Dec 16;65(1-2):109-21. doi: 10.1016/0022-1759(83)90308-3.