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来自巴西的低代次登革病毒2型的全长感染性克隆。

Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil.

作者信息

Santos Jefferson José da Silva, Magalhães Tereza, Silva Junior José Valter Joaquim, Silva Andréa Nazaré Monteiro Rangel da, Cordeiro Marli Tenório, Gil Laura Helena Vega Gonzales

机构信息

Laboratório de Virologia e Terapia Experimental, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, BR.

出版信息

Mem Inst Oswaldo Cruz. 2015 Aug;110(5):677-83. doi: 10.1590/0074-02760150053. Epub 2015 Jul 17.

DOI:10.1590/0074-02760150053
PMID:26200712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4569833/
Abstract

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.

摘要

全长登革病毒（DENV）cDNA克隆是许多研究中不可或缺的工具，包括那些关于减毒或嵌合疫苗开发以及宿主-病毒相互作用的研究。此外，低传代DENV感染性克隆的重要性应予以强调，因为这些克隆可能含有来自野外循环分离株的关键且独特的菌株特异性病毒成分。本文报道通过同源重组成功构建了功能性巴西低传代DENV血清型2全长克隆，这支持了一种已被本研究团队证明在黄病毒感染性克隆和复制子开发中非常有用的策略的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac3/4569833/46cb731e2581/0074-0276-mioc-110-5-0677-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac3/4569833/d1a9e9f6a375/0074-0276-mioc-110-5-0677-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac3/4569833/f1e958fa5d8e/0074-0276-mioc-110-5-0677-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac3/4569833/46cb731e2581/0074-0276-mioc-110-5-0677-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac3/4569833/d1a9e9f6a375/0074-0276-mioc-110-5-0677-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac3/4569833/f1e958fa5d8e/0074-0276-mioc-110-5-0677-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac3/4569833/46cb731e2581/0074-0276-mioc-110-5-0677-gf03.jpg

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