Furini Cristiane R G, Myskiw Jociane de C, Schmidt Bianca E, Zinn Carolina G, Peixoto Patricia B, Pereira Luiza D, Izquierdo Ivan
National Institute of Translational Neuroscience (INNT), National Research Council of Brazil, and Memory Center, Brain Institute of Rio Grande do Sul, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690 - 2nd Floor, 90610-000 Porto Alegre, RS, Brazil.
National Institute of Translational Neuroscience (INNT), National Research Council of Brazil, and Memory Center, Brain Institute of Rio Grande do Sul, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690 - 2nd Floor, 90610-000 Porto Alegre, RS, Brazil.
Behav Brain Res. 2015 Nov 1;294:17-24. doi: 10.1016/j.bbr.2015.07.038. Epub 2015 Jul 19.
For decades there has been a consensus that de novo protein synthesis is necessary for long-term memory. A second round of protein synthesis has been described for both extinction and reconsolidation following an unreinforced test session. Recently, it was shown that consolidation and reconsolidation depend not only on protein synthesis but also on protein degradation by the ubiquitin-proteasome system (UPS), a major mechanism responsible for protein turnover. However, the involvement of UPS on consolidation and reconsolidation of object recognition memory remains unknown. Here we investigate in the CA1 region of the dorsal hippocampus the involvement of UPS-mediated protein degradation in consolidation and reconsolidation of object recognition memory. Animals with infusion cannulae stereotaxically implanted in the CA1 region of the dorsal hippocampus, were exposed to an object recognition task. The UPS inhibitor β-Lactacystin did not affect the consolidation and the reconsolidation of object recognition memory at doses known to affect other forms of memory (inhibitory avoidance, spatial learning in a water maze) while the protein synthesis inhibitor anisomycin impaired the consolidation and the reconsolidation of the object recognition memory. However, β-Lactacystin was able to reverse the impairment caused by anisomycin on the reconsolidation process in the CA1 region of the hippocampus. Therefore, it is possible to postulate a direct link between protein degradation and protein synthesis during the reconsolidation of the object recognition memory.
几十年来，人们一直达成共识，即从头合成蛋白质对于长期记忆是必要的。在未强化的测试阶段后，已经描述了第二轮蛋白质合成与消退和重新巩固有关。最近，研究表明巩固和重新巩固不仅取决于蛋白质合成，还取决于泛素 - 蛋白酶体系统（UPS）介导的蛋白质降解，这是负责蛋白质周转的主要机制。然而，UPS在物体识别记忆的巩固和重新巩固中的作用仍然未知。在这里，我们研究了在背侧海马体的CA1区域中，UPS介导的蛋白质降解在物体识别记忆的巩固和重新巩固中的作用。将带有输注套管的动物立体定位植入背侧海马体的CA1区域，使其接受物体识别任务。UPS抑制剂β-乳酰环肽在已知会影响其他形式记忆（抑制性回避、水迷宫中的空间学习）的剂量下，并不影响物体识别记忆的巩固和重新巩固，而蛋白质合成抑制剂茴香霉素则损害了物体识别记忆的巩固和重新巩固。然而，β-乳酰环肽能够逆转茴香霉素对海马体CA1区域重新巩固过程造成的损害。因此，有可能推测在物体识别记忆的重新巩固过程中，蛋白质降解与蛋白质合成之间存在直接联系。
