Rossato Janine I, Gonzalez Maria Carolina, Souza Johseph P G, Radiske Andressa, Apolinario Gênedy, Baracho Ana Luizi, Cammarota Martín
Memory Research Laboratory, Brain Institute and Department of Physiology and Behavior, Federal University of Rio Grande do Norte, Natal, Brazil.
International Institute of Neuroscience, Macaiba, Brazil.
Front Cell Neurosci. 2025 Aug 21;19:1620588. doi: 10.3389/fncel.2025.1620588. eCollection 2025.
Object recognition memory (ORM) allows animals to distinguish between novel and familiar items. When reactivated during recall in the presence of a novel object, a consolidated ORM can be destabilized and linked to that generated by the novel object through reconsolidation. The CA1 region of the dorsal hippocampus contributes to ORM destabilization and reconsolidation, with mechanisms involving theta/gamma cross-frequency coupling (hPAC) and synaptic plasticity modulation. Ca+/calmodulin-dependent protein kinase II (CaMKII) is vital for hippocampus-dependent memory processing and has been associated with theta activity-dependent plasticity in dorsal CA1. However, the specific role of hippocampal CaMKII in the lasting storage of reactivated ORM remains unclear, and its potential impact on memory-related oscillatory activity has not been previously investigated. To explore these questions, we employed a combination of behavioral, electrophysiological, and pharmacological approaches at various stages of ORM processing, and found that CaMKII is not necessary for ORM recall or reconsolidation but does regulate novelty-induced ORM destabilization by modulating hPAC.
物体识别记忆（ORM）使动物能够区分新异和熟悉的物品。当在新异物体出现时回忆过程中被重新激活时，巩固的ORM会变得不稳定，并通过重新巩固与新异物体产生的记忆相联系。背侧海马体的CA1区域有助于ORM的不稳定和重新巩固，其机制涉及θ/γ跨频耦合（hPAC）和突触可塑性调节。钙/钙调蛋白依赖性蛋白激酶II（CaMKII）对海马体依赖的记忆处理至关重要，并且与背侧CA1中θ活动依赖性可塑性有关。然而，海马体CaMKII在重新激活的ORM的持久存储中的具体作用仍不清楚，其对记忆相关振荡活动的潜在影响此前尚未得到研究。为了探究这些问题，我们在ORM处理的各个阶段采用了行为学、电生理学和药理学相结合的方法，发现CaMKII对于ORM的回忆或重新巩固并非必需，但确实通过调节hPAC来调控新异性诱导的ORM不稳定。
