Ménoret Antoine, Crocker Stephen J, Rodriguez Annabelle, Rathinam Vijay A, Clark Robert B, Vella Anthony T
Department of Immunology, University of Connecticut Health Center, Farmington, CT, USA.
Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, USA.
Proteomics Clin Appl. 2016 Jan;10(1):8-24. doi: 10.1002/prca.201500029. Epub 2015 Sep 16.
Proteomic strategies provide a valuable tool kit to identify proteins involved in diseases. With recent progress in MS technology, high throughput proteomics has accelerated protein identification for potential biomarkers. Numerous biomarker candidates have been identified in several diseases, and many are common among pathologies. An overall strategy that could complement and strengthen the search for biomarkers is combining protein identity with biological outcomes. This review describes an emerging framework of bridging bioactivity to protein identity, exploring the possibility that some biomarkers will have a mechanistic role in the disease process. A review of pulmonary, cardiovascular, and CNS biomarkers will be discussed to demonstrate the utility of combining bioactivity with identification as a means to not only find meaningful biomarkers, but also to uncover functional mediators of disease.
蛋白质组学策略提供了一套有价值的工具，用于识别与疾病相关的蛋白质。随着质谱技术的最新进展，高通量蛋白质组学加速了对潜在生物标志物的蛋白质识别。在几种疾病中已经鉴定出了大量的生物标志物候选物，其中许多在不同病理中是常见的。一种可以补充和加强生物标志物搜索的总体策略是将蛋白质识别与生物学结果相结合。本综述描述了一个将生物活性与蛋白质识别联系起来的新兴框架，探讨了一些生物标志物在疾病过程中具有机制作用的可能性。将讨论对肺部、心血管和中枢神经系统生物标志物的综述，以证明将生物活性与识别相结合作为一种手段的实用性，不仅可以找到有意义的生物标志物，还可以揭示疾病的功能介质。
