Yang Pengyu, Liu Kai
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037 (USA).
Chembiochem. 2015 Mar 23;16(5):712-24. doi: 10.1002/cbic.201402582. Epub 2015 Feb 4.
The completion of the human genome sequencing project has provided a wealth of new information regarding the genomic blueprint of the cell. Although, to date, there are roughly 20,000 genes in the human genome, the functions of only a handful of proteins are clear. The major challenge lies in translating genomic information into an understanding of their cellular functions. The recently developed activity-based protein profiling (ABPP) is an unconventional approach that is complementary for gene expression analysis and an ideal utensil in decoding this overflow of genomic information. This approach makes use of synthetic small molecules that covalently modify a set of related proteins and subsequently facilitates identification of the target protein, enabling rapid biochemical analysis and inhibitor discovery. This tutorial review introduces recent advances in the field of ABPP and its applications.
人类基因组测序项目的完成提供了大量有关细胞基因组蓝图的新信息。尽管迄今为止人类基因组中大约有20000个基因，但只有少数蛋白质的功能是明确的。主要挑战在于将基因组信息转化为对其细胞功能的理解。最近开发的基于活性的蛋白质谱分析（ABPP）是一种非常规方法，它是基因表达分析的补充手段，也是解码这些海量基因组信息的理想工具。这种方法利用合成小分子与一组相关蛋白质进行共价修饰，随后便于鉴定目标蛋白质，从而实现快速生化分析和抑制剂发现。本教程综述介绍了ABPP领域的最新进展及其应用。
