Del Rosario Carlos, Rodríguez-Évora María, Reyes Ricardo, Delgado Araceli, Évora Carmen
Department of Chemical Engineering and Pharmaceutical Technology, University of La Laguna, 38200 La Laguna, Spain.
Biomed Mater. 2015 Jul 23;10(4):045008. doi: 10.1088/1748-6041/10/4/045008.
The aim of this work was to study the bone repair induced by bone morphogenetic protein-2 (BMP-2), rat mesenchymal stem cells (rMSCs), and platelet-derived growth factor (PDGF-BB) incorporated in a macroporous beta-tricalcium phosphate (β-TCP) system fabricated by robocasting, and to identify the most beneficial combination in a critical rat calvaria defect. BMP-2 was formulated in microspheres to provide a prolonged, local concentration, whereas PDGF-BB, which acts during the initial stage of defect repair, was incorporated in a thin layer of crosslinked alginate. Approximately 80% of PDGF-BB and 90% of BMP-2 were released into the defect during the first 2 d and 3 weeks, respectively. Histological analyses indicated a minor synergistic effect in the BMP-2-MSC groups. In contrast, significant antagonism was found with combined BMP-2 and PDGF-BB defect treatment. The high-grade repair induced by BMP-2 rules out any advantage from combining BMP-2 with PDGF-BB or MSCs, at least with this scaffold and defect model.
本研究旨在探讨骨形态发生蛋白-2（BMP-2）、大鼠间充质干细胞（rMSCs）和血小板衍生生长因子（PDGF-BB）负载于通过三维打印制造的大孔β-磷酸三钙（β-TCP）系统中所诱导的骨修复情况，并在大鼠颅骨关键缺损模型中确定最有益的组合。BMP-2被制成微球以提供持续的局部浓度，而在缺损修复初期起作用的PDGF-BB则负载于交联藻酸盐薄层中。在最初的2天和3周内，分别约80%的PDGF-BB和90%的BMP-2释放到缺损处。组织学分析表明，BMP-2-MSC组存在轻微的协同作用。相反，联合使用BMP-2和PDGF-BB治疗缺损时发现显著的拮抗作用。至少在这种支架和缺损模型中，BMP-2诱导的高级别修复排除了将BMP-2与PDGF-BB或MSCs联合使用的任何优势。
