Wang Wei, Chen Bingzhi, Zhang Lei, Yan Junjie, Lu Yuanping, Zhang Xiaoyin, Jiang Yuji, Wu Taju, van Peer Arend Frans, Li Shaojie, Xie Baogui
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2015 Jul 22;16(7):16669-82. doi: 10.3390/ijms160716669.
Molecular markers and genetic maps are useful tools in genetic studies. Novel molecular markers and their applications have been developed in recent years. With the recent advancements in sequencing technology, the genomic sequences of an increasingly great number of fungi have become available. A novel type of molecular marker was developed to construct the first reported linkage map of the edible and economically important basidiomycete Volvariella volvacea by using 104 structural variation (SV) markers that are based on the genomic sequences. Because of the special and simple life cycle in basidiomycete, SV markers can be effectively developed by genomic comparison and tested in single spore isolates (SSIs). This stable, convenient and rapidly developed marker may assist in the construction of genetic maps and facilitate genomic research for other species of fungi.
分子标记和遗传图谱是遗传学研究中的有用工具。近年来开发了新型分子标记及其应用。随着测序技术的最新进展，越来越多真菌的基因组序列已可获取。通过使用基于基因组序列的104个结构变异（SV）标记，开发了一种新型分子标记，以构建首次报道的食用且具有经济重要性的担子菌草菇的连锁图谱。由于担子菌特殊且简单的生命周期，SV标记可通过基因组比较有效开发，并在单孢子分离物（SSI）中进行测试。这种稳定、方便且快速开发的标记可能有助于构建遗传图谱，并促进其他真菌物种的基因组研究。
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