Gong Wen-Bing, Liu Wei, Lu Ying-Ying, Bian Yin-Bing, Zhou Yan, Kwan Hoi Shan, Cheung Man Kit, Xiao Yang
Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan 430070, Hubei Province, PR China; Institute of Applied Mycology, Huazhong Agricultural University, Hubei Province 430070, PR China.
Institute of Applied Mycology, Huazhong Agricultural University, Hubei Province 430070, PR China; Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Province 430072, PR China.
Fungal Biol. 2014 Mar;118(3):295-308. doi: 10.1016/j.funbio.2014.01.001. Epub 2014 Jan 21.
The most saturated linkage map for Lentinula edodes to date was constructed based on a monokaryotic population of 146 single spore isolates (SSIs) using sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), insertion-deletion (InDel) markers, and the mating-type loci. Five hundred and twenty-four markers were located on 13 linkage groups (LGs). The map spanned a total length of 1006.1 cM, with an average marker spacing of 2.0 cM. Quantitative trait loci (QTLs) mapping was utilized to uncover the loci regulating and controlling the vegetative mycelium growth rate on various synthetic media, and complex medium for commercial cultivation of L. edodes. Two and 13 putative QTLs, identified respectively in the monokaryotic population and two testcross dikaryotic populations, were mapped on seven different LGs. Several vegetative mycelium growth rate-related QTLs uncovered here were clustered on LG4 (Qmgr1, Qdgr1, Qdgr2 and Qdgr9) and LG6 (Qdgr3, Qdgr4 and Qdgr5), implying the presence of main genomic areas responsible for growth rate regulation and control. The QTL hotspot region on LG4 was found to be in close proximity to the region containing the mating-type A (MAT-A) locus. Moreover, Qdgr2 on LG4 was detected on different media, contributing 8.07 %-23.71 % of the phenotypic variation. The present study provides essential information for QTL mapping and marker-assisted selection (MAS) in L. edodes.
迄今为止，香菇最饱和的连锁图谱是基于146个单孢分离株（SSI）的单核群体构建的，使用了序列相关扩增多态性（SRAP）、靶区域扩增多态性（TRAP）、插入缺失（InDel）标记以及交配型位点。524个标记位于13个连锁群（LG）上。该图谱全长1006.1厘摩，平均标记间距为2.0厘摩。利用数量性状位点（QTL）定位来揭示在各种合成培养基以及香菇商业栽培用复合培养基上调控营养菌丝生长速率的位点。在单核群体和两个测交双核群体中分别鉴定出的2个和13个推定QTL，被定位在7个不同的LG上。这里发现的几个与营养菌丝生长速率相关的QTL聚集在LG4（Qmgr1、Qdgr1、Qdgr2和Qdgr9）和LG6（Qdgr3、Qdgr4和Qdgr5）上，这意味着存在负责生长速率调控的主要基因组区域。发现LG4上的QTL热点区域与包含交配型A（MAT-A）位点的区域紧密相邻。此外，LG4上的Qdgr2在不同培养基上均被检测到，贡献了8.07%-23.71%的表型变异。本研究为香菇的QTL定位和标记辅助选择（MAS）提供了重要信息。
