Ding Ning, Chen Qian, Xu Hai-gang, Ji Meng-meng, Jiang Han, Jiang Yuan-mao
Ying Yong Sheng Tai Xue Bao. 2015 Mar;26(3):755-60.
Five-year-old 'Fuji'3/M26/M. hupehensis Rehd. seedlings were treated by 15N tracer to study the effects of fertilization depth (0, 20 and 40 cm) on 15N-urea absorption, distribution, utilization and loss in soil. The results showed that the plant leaf area, chlorophyll content and total N of apple leaves in 20 cm treatment were obviously higher than 0 cm and 40 cm treatments. The 15N derived from fertilizer (Ndff) in different organs of apple plant under different depths were significantly different, and the Ndff was the highest in roots at the full-bloom stage, and then in perennial branches. During the shoot rapid-growing and flower bud differentiation stage, the Ndff of new organs higher than that of the storage organs, and the Ndff of different organs were high level at fruit rapid-expanding stage, and the Ndff of fruit was the highest. The distribution ratio of 15N at fruit maturity stage was significantly different under fertilization depths, and that of the vegetative and repro- ductive organs of 20 cm treatment were obviously higher than 0 cm and 40 cm treatments, but that of the storage organs of 20 cm treatment was lower than 0 cm and 40 cm treatments. At fruit maturity stage, 15N utilization rate of apple plant of 20 cm treatment was 24.0%, which was obviously higher than 0 cm (14.1%) and 40 cm (7.6%) treatments, and 15N loss rate was 54.0%, which was obviously lower than 0 cm (67.8%) and 40 cm (63.5%) treatments. With the increase of fertilization depths, the N residue in soil increased sharply.
以5年生‘富士’3/ M26/湖北海棠实生苗为试材,采用15N示踪技术,研究了施肥深度(0、20和40 cm)对土壤中15N-尿素吸收、分配、利用及损失的影响。结果表明,20 cm处理的苹果叶片面积、叶绿素含量和全氮含量显著高于0 cm和40 cm处理。不同深度下苹果植株各器官中肥料来源的15N(Ndff)差异显著,盛花期根系中Ndff最高,其次是多年生枝。新梢速长期和花芽分化期,新器官中Ndff高于贮藏器官,果实快速膨大期各器官Ndff均处于较高水平,果实中Ndff最高。果实成熟期,不同施肥深度下15N的分配比例差异显著,20 cm处理营养器官和生殖器官中15N分配比例显著高于0 cm和40 cm处理,而20 cm处理贮藏器官中15N分配比例低于0 cm和40 cm处理。果实成熟期,20 cm处理苹果植株15N利用率为24.0%,显著高于0 cm(14.1%)和40 cm(7.6%)处理,15N损失率为54.0%,显著低于0 cm(67.8%)和40 cm(63.5%)处理。随着施肥深度增加,土壤中氮素残留量急剧增加。