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小鼠脊髓室管膜的体视学研究。附脉络丛室管膜的比较说明。

A stereological study of the ependyma of the mouse spinal cord. With a comparative note on the choroid plexus ependyma.

作者信息

Bjugn R, Bøe R, Haugland H K

机构信息

Institute of Anatomy, University of Bergen, Norway.

出版信息

J Anat. 1989 Oct;166:171-8.

PMID:2621136
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1256750/
Abstract

Applying different stereological techniques, the total ependymal volume in the spinal cord of mice was estimated to be 83 x 10(6) microns cubed, the number of cells to be 163,000 and the mean ependymal cell volume to be 510 microns cubed. Compared to choroid plexus cells in the third ventricle, the ependymal cells in the spinal cord contained a smaller mitochondrial volume (9.8% versus 4.6% of cell volume) and less rough endoplasmic reticulum (2.1% versus 0.4%). These findings indicate that the metabolic activity of the ependyma in the spinal cord is lower than that in the choroid plexus. Compared to liver and exocrine pancreatic cells, ependymal cells in both locations must be considered to have a rather low metabolic activity.

摘要

应用不同的体视学技术,估计小鼠脊髓室管膜总体积为83×10⁶立方微米,细胞数量为163,000个,室管膜细胞平均体积为510立方微米。与第三脑室脉络丛细胞相比,脊髓室管膜细胞的线粒体体积较小(分别占细胞体积的9.8%和4.6%),粗面内质网较少(分别占2.1%和0.4%)。这些发现表明,脊髓室管膜的代谢活性低于脉络丛。与肝脏和胰腺外分泌细胞相比,这两个部位的室管膜细胞代谢活性都相当低。

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本文引用的文献

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POST-NATAL GROWTH OF NEUROGLIA CELLS AND BLOOD VESSELS OF THE CERVICAL SPINAL CORD OF THE ALBINO MOUSE.白化小鼠颈脊髓神经胶质细胞和血管的产后生长
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Mitochondria: a historical review.线粒体:历史回顾
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Further studies of specimen volume changes during processing for SEM: including some plant tissue.扫描电子显微镜(SEM)样品制备过程中样品体积变化的进一步研究:包括一些植物组织。
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Stereological estimation of the volume-weighted mean volume of arbitrary particles observed on random sections.对随机切片上观察到的任意颗粒的体积加权平均体积进行体视学估计。
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6
Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson.任意颗粒的体视学。对无偏数量和大小估计器的综述以及一些新估计器的介绍,以纪念威廉·R·汤普森。
J Microsc. 1986 Jul;143(Pt 1):3-45.
7
Particle number can be estimated using a disector of unknown thickness: the selector.颗粒数量可以使用厚度未知的夹层片(即选择器)来估算。
J Microsc. 1987 Feb;145(Pt 2):121-42.
8
The impact of recent stereological advances on quantitative studies of the nervous system.近期体视学进展对神经系统定量研究的影响。
J Neurosci Methods. 1986 Oct;18(1-2):39-78. doi: 10.1016/0165-0270(86)90112-3.
9
Preservation of the ependyma of the mouse spinal cord by various vascular perfusion procedures.通过各种血管灌注程序保存小鼠脊髓的室管膜。
J Neurosci Methods. 1988 Feb;23(1):49-55. doi: 10.1016/0165-0270(88)90021-0.
10
Some new, simple and efficient stereological methods and their use in pathological research and diagnosis.一些新型、简单且高效的体视学方法及其在病理学研究与诊断中的应用。
APMIS. 1988 May;96(5):379-94. doi: 10.1111/j.1699-0463.1988.tb05320.x.