Mikrobielle Wirkstoffe, Helmholtz Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig (Deutschland).
Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig (Deutschland).
Angew Chem Int Ed Engl. 2015 Sep 14;54(38):11254-8. doi: 10.1002/anie.201500927. Epub 2015 Jul 24.
Lantibiotics (lanthionine-containing antibiotics) from Gram-positive bacteria typically exhibit activity against Gram-positive bacteria. The activity and structure of pinensin A (1) and B (2), lantibiotics isolated from a native Gram-negative producer Chitinophaga pinensis are described. Surprisingly, the pinensins were found to be highly active against many filamentous fungi and yeasts but show only weak antibacterial activity. To the best of our knowledge, lantibiotic fungicides have not been described before. An in-depth bioinformatic analysis of the biosynthetic gene cluster established the ribosomal origin of these compounds and identified candidate genes encoding all of the enzymes required for post-translational modification. Additional encoded functions enabled us to build up a hypothesis for the biosynthesis, export, sensing, and import of this intriguing lantibiotic.
来自革兰氏阳性菌的类细菌素（含硫氨酸的抗生素）通常对革兰氏阳性菌具有活性。本文描述了从天然革兰氏阴性产菌几丁质分解菌 Chitinophaga pinensis 中分离出的类细菌素 pinensin A（1）和 B（2）的活性和结构。令人惊讶的是，pinensins 对许多丝状真菌和酵母表现出高度活性，但仅显示出较弱的抗菌活性。据我们所知，以前尚未描述过类细菌素杀真菌剂。对生物合成基因簇的深入生物信息学分析确定了这些化合物的核糖体起源，并鉴定了编码所有翻译后修饰所需酶的候选基因。额外编码的功能使我们能够建立关于这种有趣的类细菌素生物合成、导出、感应和导入的假设。
