Rakicka Magdalena, Lazar Zbigniew, Dulermo Thierry, Fickers Patrick, Nicaud Jean Marc
INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France ; AgroParisTech, UMR Micalis, Jouy-en-Josas, France ; Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Str. 37/41, 51-630 Wrocław, Poland ; Institut Micalis, INRA-AgroParisTech, UMR1319, Team BIMLip: Biologie Intégrative du Métabolisme Lipidique, CBAI, 78850 Thiverval-Grignon, France.
INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France ; AgroParisTech, UMR Micalis, Jouy-en-Josas, France ; Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Str. 37/41, 51-630 Wrocław, Poland.
Biotechnol Biofuels. 2015 Jul 25;8:104. doi: 10.1186/s13068-015-0286-z. eCollection 2015.
Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry.
In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculum densities was evaluated in fed-batch cultures. The greatest lipid content, 31% of CDW, was obtained using a low-density inoculum, a constant agitation rate of 800 rpm, and an oxygenation rate of 1.5 L/min. When the strain was cultured for 450 h in a chemostat containing a nitrogen-limited medium (dilution rate of 0.01 h(-1); 250 g/L crude glycerol), volumetric lipid productivity was 0.43 g/L/h and biomass yield was 60 g CDW/L. The coefficient of lipid yield to glycerol consumption (Y L/gly) and the coefficient of lipid yield to biomass yield (Y L/X ) were equal to 0.1 and 0.4, respectively.
These results indicate that lipids may be produced using renewable feedstock, thus providing a means of decreasing the cost of biodiesel production. Furthermore, using molasses for biomass production and recycling glycerol from the biodiesel industry should allow biolipids to be sustainably produced.
利用可再生原料生产微生物脂质对生物柴油行业具有巨大潜力。
在本研究中，对一株经过脂质工程改造的解脂耶氏酵母菌株JMY4086在补料分批培养中，于不同充氧条件和不同接种密度下利用糖蜜和粗甘油生产脂质的能力进行了评估。使用低密度接种物、800 rpm的恒定搅拌速率和1.5 L/min的充氧速率时，获得了最高脂质含量，占细胞干重的31%。当该菌株在含有氮限制培养基（稀释率为0.01 h(-1)；250 g/L粗甘油）的恒化器中培养450 h时，体积脂质生产率为0.43 g/L/h，生物量产量为60 g细胞干重/L。脂质产量与甘油消耗量的系数（Y L/gly）和脂质产量与生物量产量的系数（Y L/X）分别等于0.1和0.4。
这些结果表明，可以利用可再生原料生产脂质，从而提供一种降低生物柴油生产成本的方法。此外，利用糖蜜进行生物质生产并回收生物柴油行业的甘油，应能实现生物脂质的可持续生产。
