Wei Xiu Li, Lin Yan Bing, Xu Lin, Han Meng Sha, Dong Dan Hong, Chen Wei Min, Wang Li, Wei Ge Hong
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
J Basic Microbiol. 2015 Oct;55(10):1212-8. doi: 10.1002/jobm.201400940. Epub 2015 Jul 27.
A Gram-positive, facultative anaerobic, rod-shaped, and endospore-forming strain, designated 53-2(T) was isolated from the root nodule of Oxytropis ochrocephala Bunge growing on Qilian mountain, China. The strain can grow at pH 7.0-8.0, 10-50 °C and tolerate up to 11% NaCl. Optimal growth occurred at pH 7.2 and 37 °C. The result of BLASTn search based on 16S rRNA gene sequence revealed that strain 53-2(T) , being closest related to Bacillus acidicola 105-2(T) , possessed remote similarity (less than 95.64%) to the species within genus Bacillus. The DNA G + C content was 37.8%. Chemotaxonomic data (major quinone is MK-7; major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, unknown phospholipid, and aminoglycophospholipid; fatty acids are anteiso-C15: 0 , iso-C15:0 and anteiso-C17: 0 ) supported the affiliation of the isolate to the genus Bacillus. On the basis of physiological, phylogenetic, and biochemical properties, strain 53-2(T) represents a novel species within genus Bacillus, for which the name Bacillus radicibacter is proposed. The type strain is 53-2(T) (=DSM27302(T) =ACCC06115(T) =CCNWQLS5(T) ).
从生长在中国祁连山上的黄花棘豆根瘤中分离出一株革兰氏阳性、兼性厌氧、杆状且产芽孢的菌株，命名为53-2(T)。该菌株能在pH 7.0 - 8.0、10 - 50 °C条件下生长，耐受高达11%的NaCl。最适生长条件为pH 7.2和37 °C。基于16S rRNA基因序列的BLASTn搜索结果显示，菌株53-2(T)与嗜酸芽孢杆菌105-2(T)亲缘关系最近，但与芽孢杆菌属内其他种的相似性较低（小于95.64%）。DNA G + C含量为37.8%。化学分类数据（主要醌为MK-7；主要极性脂为双磷脂酰甘油、磷脂酰甘油、未知磷脂和氨基糖磷脂；脂肪酸为anteiso-C15:0、iso-C15:0和anteiso-C17:0）支持该分离株属于芽孢杆菌属。基于生理、系统发育和生化特性，菌株53-2(T)代表芽孢杆菌属中的一个新种，为此提出名为根际芽孢杆菌的名称。模式菌株为53-2(T)（=DSM27302(T)=ACCC06115(T)=CCNWQLS5(T)）。
