Li Yue-ting, Hu Jie, Xie Yu-min, Zheng Lin, Lan Yan-yu, Wang Yong-lin
Zhong Yao Cai. 2015 Jan;38(1):115-8.
To observe the effect of Polygonum orientale flower extract with different preparation methods on acute myocardial ischemia in mongrel dogs.
The acute myocardial ischemia model of dog was set up though ligation of anterior descending branch of coronary artery in dogs, then the epicardium electrocardiogram was used to measure the degree of myocardial ischemia and the quantitative histological assay was adopted to determine the area of myocardial ischemia. At the same time, the lactic dehydrogense (LDH) and creatine kinase(CK) levels in serum were analyzed. All of them were used to evaluate the protective effect of Polygonum orientale flower with different preparation methods on acute myocardial ischemia in mongrel dog.
Compared with the model group, the sample whose preparation methods was extracted by n-butanol after water extracting and alcohol precipitation showed obvious reduction in the area of myocardial ischemia,with decline in ∑ST and NST. Moreover,the LDH and CK levels in serum were decreased (P <0. 05 or P <0. 01).
The sample which is extracted by n-butanol after water extracting and alcohol precipitation has a protective effect on injury induced by acute myocardial ischemia in mongrel dog.
观察不同制备方法的红蓼花提取物对杂种犬急性心肌缺血的影响。
通过结扎犬冠状动脉前降支建立犬急性心肌缺血模型，然后采用体表心电图测量心肌缺血程度，采用定量组织学分析测定心肌缺血面积。同时，分析血清中乳酸脱氢酶（LDH）和肌酸激酶（CK）水平。所有这些均用于评价不同制备方法的红蓼花对杂种犬急性心肌缺血的保护作用。
与模型组相比，水提醇沉后正丁醇萃取制备方法的样品心肌缺血面积明显减小，∑ST和NST下降。此外，血清中LDH和CK水平降低（P<0.05或P<0.01）。
水提醇沉后正丁醇萃取制备的样品对杂种犬急性心肌缺血损伤具有保护作用。
