Broncel Malgorzata, Serwa Remigiusz A, Ciepla Paulina, Krause Eberhard, Dallman Margaret J, Magee Anthony I, Tate Edward W
Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany.
Data Brief. 2015 Jul 2;4:379-83. doi: 10.1016/j.dib.2015.06.010. eCollection 2015 Sep.
Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.
用人炔基肉豆蔻酸探针代谢标记人细胞（HEK 293、HeLa、MCF-7）和斑马鱼胚胎，用SDS缓冲液裂解，通过铜催化的炔基叠氮环加成反应（CuAAC）将标记的蛋白质组与多功能捕获试剂连接。通过提供修饰位点的直接串联质谱证据，分别在人细胞和斑马鱼中实现了对87个和61个共翻译豆蔻酰化蛋白质的亲和富集和高可信度鉴定。数据已存入蛋白质组交换联盟（Vizcaíno等人，2014年，《自然生物技术》，32卷，223 - 226页）（PXD001863和PXD001876），并在Broncel等人发表于《德国应用化学》的“用于人细胞中蛋白质修饰的定量蛋白质组全分析和脊椎动物发育过程中动态蛋白质脂酰化的多功能试剂”一文中有详细描述。
