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用于人类细胞中蛋白质修饰的全蛋白质组定量分析以及脊椎动物发育过程中蛋白质脂化动态分析的多功能试剂。

Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development.

作者信息

Broncel Malgorzata, Serwa Remigiusz A, Ciepla Paulina, Krause Eberhard, Dallman Margaret J, Magee Anthony I, Tate Edward W

机构信息

Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ (UK).

出版信息

Angew Chem Int Ed Engl. 2015 May 11;54(20):5948-51. doi: 10.1002/anie.201500342. Epub 2015 Mar 25.

DOI:10.1002/anie.201500342
PMID:25807930
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4471546/
Abstract

Novel multifunctional reagents were applied in combination with a lipid probe for affinity enrichment of myristoylated proteins and direct detection of lipid-modified tryptic peptides by mass spectrometry. This method enables high-confidence identification of the myristoylated proteome on an unprecedented scale in cell culture, and allowed the first quantitative analysis of dynamic changes in protein lipidation during vertebrate embryonic development.

摘要

新型多功能试剂与脂质探针联合应用，用于肉豆蔻酰化蛋白质的亲和富集以及通过质谱直接检测脂质修饰的胰蛋白酶肽段。该方法能够在细胞培养中以前所未有的规模对肉豆蔻酰化蛋白质组进行高可信度鉴定，并首次对脊椎动物胚胎发育过程中蛋白质脂化的动态变化进行定量分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f89/4471546/75bf91e52375/anie0054-5948-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f89/4471546/2c3d1059a071/anie0054-5948-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f89/4471546/75bf91e52375/anie0054-5948-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f89/4471546/2c3d1059a071/anie0054-5948-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f89/4471546/75bf91e52375/anie0054-5948-f2.jpg

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ProteomeXchange provides globally coordinated proteomics data submission and dissemination.蛋白质组学交换库提供全球协调的蛋白质组学数据提交和传播服务。
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